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Allophycocyanin apc conjugated anti mouse rat cd62p

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Allophycocyanin (APC) conjugated anti-mouse/rat CD62P is a flow cytometry reagent. It is used to detect and quantify the expression of CD62P, also known as P-selectin, on the surface of mouse or rat cells.

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Lab products found in correlation

Macsquant analyzer 10, by Miltenyi Biotec (3 mentions) Anti mouse cd41, by BioLegend (1 mentions)

3 protocols using allophycocyanin apc conjugated anti mouse rat cd62p

1

Quantifying Platelet Activation by Flow

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Freshly isolated platelets (106) were resuspended in 50 µL of Tyrode’s buffer. Platelets were incubated (RT for 30 min) with fluorescein isothocyanate (FITC) -conjugated anti-mouse CD41 (Clone MWReg30, BioLegend, San Diego, CA, USA) and Allophycocyanin (APC) conjugated anti-mouse/rat CD62P (Clone RMP-1, Biolegend). A minimum of 10,000 events per gate were acquired using a MACsQuant Analyzer 10 (Miltenyi Biotec, Auburn, CA, USA) and analyzed using FlowLogic software (Innovai, Sydney, Australia). Platelets were distinguished by specific binding of anti-CD41 and characteristic forward and side scattering. Platelets staining positive for CD41 and CD62P were designated as activated platelets.
Borges-Rodriguez M., Shields C.A., Travis O.K., Tramel R.W., Baik C.H., Giachelli C.A., Tardo G.A., Williams J.M, & Cornelius D.C. (2021). Platelet Inhibition Prevents NLRP3 Inflammasome Activation and Sepsis-Induced Kidney Injury. International Journal of Molecular Sciences, 22(19), 10330.
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2

Flow Cytometric Analysis of Platelet Activation

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Freshly isolated or LPS stimulated platelets (106) were resuspended in 50 μL of Tyrode's buffer. Platelets were incubated (RT for 30 min) with fluorescein isothocyanate (FITC) ‐conjugated anti‐mouse CD41 (Clone MWReg30, BioLegend, San Diego, CA) and Allophycocyanin (APC) conjugated anti‐mouse/rat CD62P (Clone RMP‐1, Biolegend). A minimum of 10,000 events per gate were acquired using a MACsQuant Analyzer 10 (Miltenyi Biotec, Auburn, CA) and analyzed using FlowLogic software (Innovai, Sydney, Australia). Platelets were distinguished by specific binding of anti‐CD41 and characteristic forward and side scattering. Platelets staining positive for CD41 and CD62P were designated as activated platelets.
Cornelius D.C., Baik C.H., Travis O.K., White D.L., Young C.M., Austin Pierce W., Shields C.A., Poudel B, & Williams J.M. (2019). NLRP3 inflammasome activation in platelets in response to sepsis. Physiological Reports, 7(9), e14073.
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3

Platelet Activation Assessment by Flow Cytometry

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Freshly isolated platelets (106) were resuspended in 50 μL of Tyrode’s buffer. Platelets were incubated (RT for 30 minutes) with fluorescein isothocyanate (FITC) -conjugated anti-mouse CD41 (Clone MWReg30, BioLegend, San Diego, CA) and Allophycocyanin (APC) conjugated anti-mouse/rat CD62P (Clone RMP-1, Biolegend). A minimum of 10,000 events per gate were acquired using a MACsQuant Analyzer 10 (Miltenyi Biotec, Auburn, CA) and analyzed using FlowLogic software (Innovai, Sydney, Australia). Platelets were distinguished by specific binding of anti-CD41 and characteristic forward and side scattering. Platelets staining positive for CD41 and CD62P were designated as activated platelets.
Cornelius D.C., Travis O.K., Tramel R.W., Borges-Rodriguez M., Baik C.H., Greer M., Giachelli C.A., Tardo G.A, & Williams J.M. (2020). NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture. PLoS ONE, 15(6), e0234039.
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