Harvested cells from spleen, bone marrow or blood were resuspended in cell staining buffer (Biolegend) and incubated with 2 μg/100μl of human anti-3BNC117 and, where applicable as indicated in the legend, with 1 μg/100μl of TruStain FcX (Biolegend) for 10 minutes, washed and resuspended again in cell staining buffer containing conjugated primary antibodies. A list of antibodies and respective dilutions used in these experiments can be found in Supplementary Table 1 . Secondary staining was performed in the dark, for 15 minutes, with anti-human IgG1 AF647 (Abcam) or anti-human IgK BV421 (Biolegend) or anti-human IgG1 FITC (Biolegend). For primary gp120 staining, cells were incubated with 2 μg/100μl of gp120. Then, cells were washed and data acquisition was performed on a CytoFLEX (Beckman Coulter) or Attune NxT (life Technologies) or FACS Aria III (BD Biosciences) for experiments involving cell sorting. Data collection was performed with CytExpert. Data were compiled and analyzed using Kaluza Analysis 2.1 (Beckman Coulter). Gating strategies can be found in Extended Data Fig. 9 .
Anti human igg1 fitc
Manufactured by BioLegend
Anti-human IgG1 FITC is a fluorescently-labeled antibody that binds to the IgG1 subclass of human immunoglobulin G. It is used for the detection and quantification of IgG1 in various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Facsaria 3, by BD (2 mentions)
Attune nxt, by Thermo Fisher Scientific (2 mentions)
Trustain fcx, by BioLegend (2 mentions)
Kaluza analysis 2, by Beckman Coulter (2 mentions)
Cell staining buffer, by BioLegend (2 mentions)
Cytoflex, by Beckman Coulter (2 mentions)
Anti human igg1 af647, by Abcam (2 mentions)
Anti human igk bv421, by BioLegend (2 mentions)
2 protocols using anti human igg1 fitc
1
Flow Cytometry Analysis of Antibody Binding
2
Flow Cytometry Analysis of Antibody Binding
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Harvested cells from spleen, bone marrow or blood were resuspended in cell staining buffer (Biolegend) and incubated with 2 μg/100μl of human anti-3BNC117 and, where applicable as indicated in the legend, with 1 μg/100μl of TruStain FcX (Biolegend) for 10 minutes, washed and resuspended again in cell staining buffer containing conjugated primary antibodies. A list of antibodies and respective dilutions used in these experiments can be found in Supplementary Table 1 . Secondary staining was performed in the dark, for 15 minutes, with anti-human IgG1 AF647 (Abcam) or anti-human IgK BV421 (Biolegend) or anti-human IgG1 FITC (Biolegend). For primary gp120 staining, cells were incubated with 2 μg/100μl of gp120. Then, cells were washed and data acquisition was performed on a CytoFLEX (Beckman Coulter) or Attune NxT (life Technologies) or FACS Aria III (BD Biosciences) for experiments involving cell sorting. Data collection was performed with CytExpert. Data were compiled and analyzed using Kaluza Analysis 2.1 (Beckman Coulter). Gating strategies can be found in Extended Data Fig. 9 .
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