Field emission scanning electron microscope

Manufactured by TESCAN

Sourced in Czechia

The Field Emission Scanning Electron Microscope (FE-SEM) is an advanced imaging tool that uses a focused beam of high-energy electrons to produce detailed, high-resolution images of a sample's surface. The FE-SEM generates images by scanning the sample with the electron beam and detecting the signals that are emitted, providing information about the sample's topography, composition, and other characteristics.

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Lab products found in correlation

Cary 100 spectrophotometer, by Agilent Technologies (1 mentions) Asap 2020, by Micromeritics (1 mentions)

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Scanning electron microscope (22 citations) MIRA3 field emission scanning electron microscope (5 citations) Mira3 XMU Field Emission Scanning Electron Microscope/SEM (2 citations)

5 protocols using field emission scanning electron microscope

Characterization of n-HA/Fe2O3/PVA Hydrogel

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The n-HA/Fe₂O₃/PVA composite hydrogel was frozen
and dried. Next, after metal spraying, scanning analysis of the surface
and cross section of the n-HA/Fe₂O₃/PVA composite
hydrogel were conducted. The microscopic morphology of the n-HA/Fe₂O₃/PVA composite hydrogel was observed and analyzed
using a TESCAN (Czech Republic) field emission scanning electron microscope.
We acquired secondary electron images and backscattered electron images.

Huang J., Liang Y., Jia Z., Chen J., Duan L., Liu W., Zhu F., Liang Q., Zhu W., You W., Xiong J, & Wang D. (2018). Development of Magnetic Nanocomposite Hydrogel with Potential Cartilage Tissue Engineering. ACS Omega, 3(6), 6182-6189.

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Fibrin Clot Formation and Ultrastructural Analysis

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Fibrin clots were induced to form directly in a microwell plate by addition of 1 μL human thrombin (3 NIH U/mL final concentration) to 33 μL plasma from either the patient or a healthy donor, and the clotting mixture was incubated at room temperature for 120 minutes. The clots were washed 3 times with 0.1 mol/L PBS (pH 7.4) and fixed with 3% glutaraldehyde in the same buffer for 4°C overnight, then dehydrated in a graded series of ethanol solutions (50%, 70%, 80%, 90%, and 100% concentrations) twice. After the 100% ethanol step, samples were immersed in hexamethyldisilazane 3 times, followed by air-drying overnight in a fume hood. The clots were mounted on aluminum stubs and sputter-coated with 10 nm gold. Fibrin clots were examined on field emission scanning electron microscope (Tescan, Brno, Czech Republic), as described previously.^{[5 (link)]}

Luo M., Deng D., Xiang L., Cheng P., Liao L., Deng X., Yan J, & Lin F. (2016). Three cases of congenital dysfibrinogenemia in unrelated Chinese families: heterozygous missense mutation in fibrinogen alpha chain Argl6His. Medicine, 95(39), e4864.

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Preparation and Characterization of PLLA/n-HA Scaffold

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The three-dimensional porous PLLA/n-HA composite scaffold was prepared by brittle fracture under low temperatures and coated with gold for SEM imaging. The microstructure of the sample was then observed by a field emission scanning electron microscope (Tescan, Czech) provided by the Research Institute of Tsinghua University in Shenzhen Micro/Nano Engineering Key Lab.

Huang J., Xiong J., Liu J., Zhu W., Chen J., Duan L., Zhang J, & Wang D. (2015). Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold. Bio-medical materials and engineering, 26 Suppl 1.

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Comprehensive Characterization of Nanocomposite Materials

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FTIR spectra were obtained with a Shimadzu-8400S (Japan) spectrometer in the wavenumber range of 400–4000 cm⁻¹; an X-ray diffractometer (Philips X-Pert 8440) with Cr Kα radiation (λ = 2.289 Å) was employed for X-ray diffraction (XRD) analysis of the powder samples. The shapes and morphologies of the samples were perceived by a MIRA3 TESCAN field emission scanning electron microscope (FESEM) armed with a link energy-dispersive X-ray (EDX) analyzer. BET measurements were performed to determine the specific surface areas and porosities of the samples with N₂ adsorption measurements at 77 K using a BELSORP Mini instrument. The samples were degassed at 180 °C prior to the nitrogen adsorption measurements. An adsorption isotherm was considered to determine the pore size distribution by the Barrett–Joyner–Halenda (BJH) method. A Raman microscope (Senterra 2009, Germany) equipped with a laser wavelength of 758 nm was employed to obtain the Raman spectra. The adsorption mechanisms of the dyes were investigated at room temperature on a Varian Cary 100 spectrophotometer. The content of P₂Mo₁₈ in the nanocomposite was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES, model OEC-730).

Hoseini A.A., Farhadi S., Zabardasti A, & Siadatnasab F. (2020). An organic–inorganic hybrid nanomaterial composed of a Dowson-type (NH4)6P2Mo18O62 heteropolyanion and a metal–organic framework: synthesis, characterization, and application as an effective adsorbent for the removal of organic dyes. RSC Advances, 10(66), 40005-40018.

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Carbonized Precursor Characterization

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All materials were purchased from Merck and Sigma-Aldrich companies and applied without further purification. The ultrasonic bath was used by Becker vCLEAN company. The furnace from the Exciton Company was used to carbonize carbon precursors. FT-IR, ¹³C-NMR, and ¹H-NMR spectra were taken by Shimadzu Fourier and Varian INOVA 500 MHz spectrum. The 9100 electrothermal devices were used for measuring melting points. The electron images were obtained by TE-SCAN Field Emission Scanning Electron Microscope. TESCAN VEGA//XMU, Philips PW1730, micromeritics ASAP 2020, STA6000, performed the EDX, XRD, BET, and TGA analyses.

Ghafuri H., Hanifehnejad P., Rashidizadeh A., Tajik Z, & Dogari H. (2023). Synthesis and characterization of nanocatalyst Cu2+/mesoporous carbon for amidation reactions of alcohols. Scientific Reports, 13, 10133.

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