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Anti act1

Manufactured by Abcam
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Anti-Act1 is a primary antibody that recognizes the Act1 protein. Act1 is a signaling adaptor protein that plays a role in the regulation of inflammatory and immune responses. Anti-Act1 can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the Act1 protein.

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Lab products found in correlation

Anti vdac1, by Abcam (2 mentions) Anti mbp, by New England Biolabs (2 mentions) Anti coq1, by Abcam (2 mentions) Irdye 800cw, by LI COR (2 mentions) Irdye 680rd goat anti mouse, by LI COR (2 mentions) Anti flag, by Merck Group (2 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions) Irdye 680rd goat anti rabbit, by LI COR (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Mouse anti-Act1 antibody

2 protocols using anti act1

1

Antibody Validation for Protein Analysis

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Primary antibodies for this study include anti-FLAG (Sigma F1804, 1:2500–1:5000), anti-Act1 (Abcam ab8224, 1:5000), anti-Coq1 (custom made at Genscript, 1:2000), anti-Vdac1 (Abcam ab110326, 1:4000), and Anti-MBP (NEB E8032S, 1:10000). Secondary antibodies include IRDye 680RD goat anti-rabbit (LI-COR 926–68071, 1:15000), IRDye 680RD goat anti-mouse (LI-COR 926–68070, 1:15000), IRDye 800CW (LI-COR 926–32210, 1:15000), and anti-mouse IgG HRP-linked (Cell Signaling Technology #7076, 1:2000–1:100000).
Tai J., Guerra R.M., Rogers S.W., Fang Z., Muehlbauer L.K., Shishkova E., Overmyer K.A., Coon J.J, & Pagliarini D.J. (2023). Hem25p is a mitochondrial IPP transporter. bioRxiv.
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2

Comprehensive Antibody Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies for this study include anti-FLAG (Sigma F1804, 1:2,500–1:5,000), anti-FLAG (Cell Signaling Technology #14793, 1:1,000), anti-Act1 (Abcam ab8224, 1:5,000), anti-Coq1 (custom made at Genscript, 1:2,000), anti-Vdac1 (Abcam ab110326, 1:4,000–1:5,000) and anti-MBP (NEB E8032S, 1:10,000). Secondary antibodies include IRDye 680RD goat anti-rabbit (LI-COR 926-68071, 1:15,000), IRDye 680RD goat anti-mouse (LI-COR 926-68070, 1:15,000), IRDye 800CW (LI-COR 926-32210, 1:15,000), anti-mouse immunoglobulin-G (IgG) horseradish peroxidase (HRP)-linked (Cell Signaling Technology #7076, 1:2,000-1:100,000) and anti-rabbit IgG HRP-linked (Cell Signaling Technology #7074, 1:20,000–1:100,000).
Tai J., Guerra R.M., Rogers S.W., Fang Z., Muehlbauer L.K., Shishkova E., Overmyer K.A., Coon J.J, & Pagliarini D.J. (2023). Hem25p is required for mitochondrial IPP transport in fungi. Nature cell biology, 25(11), 1616-1624.
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