All the strains were submitted to biochemical identification using the API Listeria system (BioMérieux, Marcy l’Etoile, France).
Api listeria system
Manufactured by bioMérieux
Sourced in France
The API Listeria system is a biochemical identification test for the detection and identification of Listeria species in food and environmental samples. It provides a rapid and accurate method for the identification of Listeria isolates.
Automatically generated - may contain errors
Lab products found in correlation
Listeria selective supplement, by Thermo Fisher Scientific (1 mentions)
Chromogenic listeria agar, by Thermo Fisher Scientific (1 mentions)
Fraser supplement, by Thermo Fisher Scientific (1 mentions)
Palcam agar, by Thermo Fisher Scientific (1 mentions)
Fraser broth, by Thermo Fisher Scientific (1 mentions)
Listeria differential supplement, by Thermo Fisher Scientific (1 mentions)
Api 20e kit, by bioMérieux (1 mentions)
3 protocols using api listeria system
1
Listeria Strain Identification
2
Listeria monocytogenes Detection in Cheese
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In accordance with ISO 11290-1:2004, detection of L. monocytogenes was carried out by suspending 10 g of cheese in 90 mL of Fraser Broth (CM0895, Oxoid Ltd.) with Half Fraser Supplement (SR0166, Oxoid Ltd.) and incubating for 24 h at 30°C. Subsequently, 0.1 mL of the first enrichment broth was suspended in 10 mL of Fraser Broth (CM0895, Oxoid Ltd.) with Fraser Supplement (SR0156, Oxoid Ltd.) and then incubated for 24 h at 37°C. One loopfull of the enrichment broth was then streaked onto PALCAM Agar (CM0877, Oxoid Ltd.) added with PALCAM Selective Supplement (SR0150, Oxoid Ltd.) and onto Chromogenic Listeria Agar (CM1084, Oxoid Ltd.) supplemented with Listeria Selective Supplement (SR0226, Oxoid Ltd.) and Listeria Differential Supplement (SR0244, Oxoid Ltd.). Both plates were incubated for 48 h at 37°C, then Listeria monocytogenes presumptive colonies were biochemically identified by using the API Listeria System according to manufacturer's instructions (bioMérieux).
3
Bacterial Identification Protocol using Biochemical Tests
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The bacterial colonies isolated on different kinds of selective media were subjected to the purification step. Preliminary identification of isolates was performed using Gram staining, coagulase test, catalase test, indole production test, methyl red test, Voges-Proskauer test, urease test, citrate utilization test, and sugar fermentation test (glucose, lactose, rhamnose, and fructose), and oxidase test according to Bergey's Manual of Systematic Bacteriology (Holt & Williams, 1989; Holt et al., 1994; Harrigan, 1998) . In the case of L. monocytogenes., the API Listeria system (BioMerieux, La Balme-Les-Grottes, France) was used as a confirmatory test (Setiani et al., 2015) . Additionally, the identification of Gram-negative bacterial isolates was confirmed by the API 20E kit (BioMerieux, La Balme-Les-Grottes, France) following the manufacturer's instructions. An aliquot of 0.1mL of a suspension of each isolate was inoculated in each microtubule of the APIkit strip and incubated for 24-48hrs at 37 0 C. After incubation, the result of each reaction was identified using the Catalogue Analytique.
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