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Human thrombin antithrombin complex elisa kit

Manufactured by Abcam
Sourced in Canada

The Human Thrombin-Antithrombin Complex ELISA Kit is a quantitative assay designed to measure the levels of the thrombin-antithrombin complex in human samples. This complex is formed when thrombin, a serine protease involved in blood coagulation, binds to antithrombin, a serpin that inhibits thrombin activity.

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3 protocols using human thrombin antithrombin complex elisa kit

1

Quantifying Plasma Coagulation Markers

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We assessed plasma samples by using commercially available kits. For plasminogen activator inhibitor 1 (PAI-1) we used Human Serpin E1/PAI-1 Quantikine ELISA Kit (R&D Systems, https://www.rndsystems.com) or Human PAI-1 Platinum Kit (eBioscience, https://www.thermofisher.com). We used Human t-Plasminogen Activator/tPA Quantikine ELISA Kit (R&D Systems) to measure tissue plasminogen activator (tPA). We used Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit (R&D Systems) or Human Thrombomodulin ELISA Kit (Innovative Research Inc., https://www.innov-research.com) to measure thrombomodulin (THBD) and Human Thrombin-Antithrombin Complex ELISA Kit (Abcam, https://www.abcam.com) to measure thrombinantithrombin (TAT) complexes. To assess endothelial protein C receptor (EPCR) we used Human EPCR DuoSet ELISA Kit (R&D Systems), for D-dimer we used Human D-Dimer ELISA Kit (Abcam), for a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS-13) we used Human ADAMTS13 Quantikine ELISA Kit (R&D Systems), for P-selectin we used Human CD62P ELISA Kit (Abcam), for hepatocyte growth factor (HGF) we used Human HGF Quantikine ELISA Kit (R&D Systems), for von Willebrand factor (vWF) we used vWF Human ELISA Kit (ThermoFisher, https://www.thermofisher.com), and for tissue factor we used Human Coagulation Factor III/Tissue Factor Quantikine ELISA Kit (R&D Systems).
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2

Perioperative Coagulation Biomarkers

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Peripheral blood samples were drawn at the following time points: 1–3 days prior to operation (T1), second hour (T2), first (T3) and third day (T4) after the operation, respectively. All samples were placed on ice and centrifuged at 3500 rpm for 5 min. The plasma was frozen and stored at − 80 °C. Preoperative and postoperative samples were drawn to assess plasma D-dimer levels by the central laboratory of our hospital. Plasma PAI-1, TAT and F1 + 2 levels were measured by enzyme linked immunosorbent assay (ELISA) using the Human PAI1 ELISA Kit (Abcam Inc, Toronto, ON, Canada), Human Thrombin-Antithrombin Complex ELISA Kit (Abcam Inc, Toronto, ON, Canada) and Human Prothrombin Fragment 1 + 2 ELISA Kit (Novus Biologicals, CO, USA), respectively.
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3

Quantifying Coagulation Markers

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D-dimer levels were measured using a latex- immunoturbidimetric assay (STA-Liatest D-Di/Diagnostica Stago). Thrombin-antithrombin complexes, were measured by ELISA following the manufacturer’s instructions (Human thrombin-antithrombin complex ELISA kit, no. ab108907, Abcam,).
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