In situ tensile straining tests were carried out in an aberration-corrected TEM operated at 300 kV (JEM-ARM300CF, JEOL) equipped with a straining holder (Model 654, Gatan). In situ TEM movies were recorded with a high-speed camera at a frame rate of 50 frames per second and 2k resolution (Oneview, Gatan). The displacement of the whole ~9 mm large sample support was controlled by a step motor whose resolution was 1 μm. The straining was interrupted intermittently to stabilize the stage and observe dislocation glide motion. No quantitative load-displacement data can be obtained during the deformation.
The contrast of dislocations in TEM images and videos is enhanced by image processing. Firstly, the background noise from the FIB damage is removed by using direct subtraction of the last frame after aligning all frames using the cross-correlation method. Then, a Gaussian filter is used to blur the remaining noise. To extract dislocation positions as shown in Fig.1b , a ridge detection filter is used after applying an additional Gaussian filter.
The contrast of dislocations in TEM images and videos is enhanced by image processing. Firstly, the background noise from the FIB damage is removed by using direct subtraction of the last frame after aligning all frames using the cross-correlation method. Then, a Gaussian filter is used to blur the remaining noise. To extract dislocation positions as shown in Fig.