Methyl lycaconitine citratehydrate mla

Manufactured by Merck Group

Sourced in United States

Methyl lycaconitine citratehydrate (MLA) is a laboratory chemical compound. It is a salt containing the organic compound methyllycaconitine and citric acid. MLA is commonly used as a research tool in scientific studies.

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Lab products found in correlation

Milli q purification system, by Merck Group (1 mentions) Nicotine nic, by Merck Group (1 mentions) Nicotine, by Merck Group (1 mentions) Ng nitro l arginine l nna, by Merck Group (1 mentions)

2 protocols using methyl lycaconitine citratehydrate mla

Cytotoxicity Evaluation of Drug Compounds

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MAN (purity > 98%) was purchased from SanHerb Bioscience Inc. (Chengdu,
Sichuan, China). Lipopolysaccharide (LPS, from gram-negative bacteria E.
coli 055:B5), nicotine (Nic) and methyl lycaconitine citrate
hydrate (MLA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal
bovine serum (FBS), RMPI-1640 medium, phosphate buffered saline (PBS),
penicillin-streptomycin together with enhanced chemiluminescence (ECL) detection
kit were supplied by Thermo Scientific (Rockford, IL, USA). Antibodies and BCA
protein quantitative kit were purchased from Cell Signaling Technology (Beverly,
MA, USA) and Keygen Biotech (Nanjing, Jiangsu, China), respectively. Ultra-pure
water was prepared by using a Milli-Q purification system (Millipore, Bedford,
MA, USA).

Yang Z., Yin Q., Olatunji O.J., Li Y., Pan S., Wang D.D, & Zuo J. (2020). Activation of cholinergic anti-inflammatory pathway involved in therapeutic actions of α-mangostin on lipopolysaccharide-induced acute lung injury in rats. International Journal of Immunopathology and Pharmacology, 34, 2058738420954941.

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Pharmacological Modulation of Cerebellar Signaling

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The reagents included picrotoxin, D-(-)-2-amino-5-phosphonopentanoic acid (D-APV), NG-Nitro-L-arginine (L-NNA), nitric oxide synthase (NOS) inhibitor, nicotine, methyllycaconitine citrate hydrate (MLA), and dihydro-β-erythroidine hydrobromide (DβEH) bought from Sigma-Aldrich (Shanghai, China). All these chemicals were dissolved in solution and kept in frozen aliquots. For experiments with NOS inhibitor, L-NNA, cerebellar surface was superfused with 200 μM L-NNA for 1 h before recordings were began. The drugs were dissolved in ACSF and applied directly onto the cerebellar surface by a peristaltic pump (0.5 ml/min).

Cao L.X., Bing Y.H., Xu Y.H., Zhang G.J., Chu C.P., Hong L, & Qiu D.L. (2022). Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice. Frontiers in Cellular Neuroscience, 16, 905724.

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