Primary and secondary lipid oxidation products were quantified by determination of peroxide value (PV) and 2-thiobarbituric acid reactive substances (TBARs). According to Crowe and White [57 (link)], PV was determined and the results were expressed in meq active O2/kg oil [58 (link)]. TBARs assay was performed following the procedure of Mozzon and Frega [59 ]. The optical density of the prepared solution was measured at 532 nm by using a Varian Cary 5000 UV–Vis–NIR spectrophotometer (Agilent, Santa Clara, CA, USA). The results were expressed in μmol TBARS/g oil.
Varian cary 5000 uv vis nir spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States
The Varian Cary 5000 UV–Vis–NIR spectrophotometer is a laboratory instrument designed to measure the absorption, transmission, or reflectance of light in the ultraviolet, visible, and near-infrared regions of the electromagnetic spectrum. It provides high-performance and versatile spectroscopic capabilities for a wide range of applications.
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Lab products found in correlation
Biologic sp50, by Bio-Logic (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Miniflex 2, by Rigaku (1 mentions)
S 4300, by Hitachi (1 mentions)
F 700, by Hitachi (1 mentions)
Spectrometers, by Bruker (1 mentions)
Waters 2695 hplc, by Waters Corporation (1 mentions)
Fts ft ir spectrophotometer, by PerkinElmer (1 mentions)
Silica gel 60, by Silicycle (1 mentions)
Silica gel 100, by Silicycle (1 mentions)
Varian cary eclipse, by Agilent Technologies (1 mentions)
5 protocols using varian cary 5000 uv vis nir spectrophotometer
1
Lipid Oxidation Assessment Protocol
2
Electrochemical and Optical Analysis of PEDOT:PSS
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Concerning the PEDOT:PSS single layer on ITO/glass study, the electrochemical analyses were carried out in a three-electrode cell configuration using a BioLogic SP50 potentiostat/galvanostat apparatus (BioLogic SP50, Seyssinet Pariset, France). The counter electrode and reference electrode consisted of a platinum foil and saturated calomel electrode, SCE (ESCE = 0.234 V/NHE), respectively. The operating voltage was controlled between −1.3 V and +1.3 V for chronoamperometry analysis, in a commercial lithium-based ionic liquid, namely EmimTFSI:LiTFSI (9:1 Molar Ratio, Solvionic, 99.9%). All the electrochemical measurements were performed at room temperature. The optical transmittance of PEDOT:PSS thin films was measured in situ using a Varian Cary 5000 UV-vis-NIR spectrophotometer (Agilent, les Ulis, France) between 250 and 800 nm.
Concerning the PEDOT:PSS displays, electrochemical analysis was performed in a two-electrode configuration, using a BioLogic SP50 potentiostat/galvanostat apparatus (BioLogic SP50, Seyssinet Pariset, France). Colorimetry analysis was carried out using a Konica Minolta CM-700D spectrophotometer (Konica Minolta Sensing Europe B.V., Roissy, France), allowing the direct determination of colorimetric parameters of the CIE (L*a*b*) color space.
Concerning the PEDOT:PSS displays, electrochemical analysis was performed in a two-electrode configuration, using a BioLogic SP50 potentiostat/galvanostat apparatus (BioLogic SP50, Seyssinet Pariset, France). Colorimetry analysis was carried out using a Konica Minolta CM-700D spectrophotometer (Konica Minolta Sensing Europe B.V., Roissy, France), allowing the direct determination of colorimetric parameters of the CIE (L*a*b*) color space.
3
Characterization of Synthesized Nanoparticles
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The crystal structure
of the synthesized particles was investigated with X-ray diffraction
(XRD, Rigaku MiniFlex-II desktop X-ray diffractometer, Cu Kα
radiation, λ = 1.5406 Å). The morphology of the particles
and the as-made films was observed by using scanning electron microscope
(SEM, Hitachi S-4300) and transmission electron microscope (TEM, JEOL,
JEM-2100F, operated at 200 keV). To obtain the emission spectra of
YSO nanoparticle powder, UCM layer, and UCM–PCM hybrid film,
fluorescence spectrophotometer (Hitachi F-700) was used under excitation
wavelength of 505 nm. The function of the UCM film on the PCM film
was investigated using solar simulated light source (Asahi HAL-320,
100 mW cm–2). The solar light was illuminated with
solar simulator with different illumination times on the area of 1
cm2. The optical properties were studied with Varian Cary
5000 UV–vis–NIR Spectrophotometer (Agilent Technologies).
of the synthesized particles was investigated with X-ray diffraction
(XRD, Rigaku MiniFlex-II desktop X-ray diffractometer, Cu Kα
radiation, λ = 1.5406 Å). The morphology of the particles
and the as-made films was observed by using scanning electron microscope
(SEM, Hitachi S-4300) and transmission electron microscope (TEM, JEOL,
JEM-2100F, operated at 200 keV). To obtain the emission spectra of
YSO nanoparticle powder, UCM layer, and UCM–PCM hybrid film,
fluorescence spectrophotometer (Hitachi F-700) was used under excitation
wavelength of 505 nm. The function of the UCM film on the PCM film
was investigated using solar simulated light source (Asahi HAL-320,
100 mW cm–2). The solar light was illuminated with
solar simulator with different illumination times on the area of 1
cm2. The optical properties were studied with Varian Cary
5000 UV–vis–NIR Spectrophotometer (Agilent Technologies).
4
Spectroscopic Characterization of Compounds
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Melting points were measured with a Büchi B-540 melting point apparatus (Flawil, Sankt Gallen, Switzerland). UV–vis spectra were recorded with a Varian Cary 5000 UV–vis–NIR spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The IR spectra were recorded using a Perkin-Elmer FTS FT-IR spectrophotometer (Waltham, MA, USA). The NMR spectra were measured using 400, 500, or 600 MHz Bruker spectrometers (Billerica, MA, USA). HRESITOFMS and LRESIMS spectra were carried out on a Bruker-Hewlett-Packard 1100 Esquire-LC system mass spectrometer (Billerica, MA, USA) and Waters 2695 HPLC (Milford, MA, USA), Waters ZQ equipped with ESCI ion source mass spectrometer, respectively. All quick column chromatography (QCC) and column chromatography (CC) were carried out on silica gel 60 (5–40 μm, SiliCycle Inc., Québec, QC G1P 4S6, Canada) and silica gel 100 (63–200 μm, SiliCycle Inc.), respectively. Sephadex LH-20, when indicated, was also used for CC. Precoated thin-layer chromatography (TLC) plates of silica gel 60 F254 were used for analytical purposes.
5
Characterization of DiI/DiD-DELOS nanovesicles
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Absorption spectra were recorded using a Varian Cary 5000 UV-Vis-NIR spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) while for fluorescence emission and excitation spectra collection, a Varian Cary Eclipse equipment (Agilent Technologies, Santa Clara, CA, USA) was used. Fluorescence spectra were collected at 490 nm and the emission were collected at 500–800 nm for DiI/DiD-DELOS nanovesicles. In all the cases, samples were diluted 10 times in water and placed in a 1 cm quartz cuvette.
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