Oxygen consumption assay kit

Manufactured by Abcam

The Oxygen Consumption Assay Kit is a laboratory tool designed to measure the oxygen consumption rate (OCR) of cells or tissues. It provides a reliable method to quantify cellular respiratory activity under various experimental conditions.

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Lab products found in correlation

Infinite m200 pro, by Tecan (2 mentions) Microplate reader, by Agilent Technologies (1 mentions) Fatty acid oxidation assay kit, by Abcam (1 mentions)

Close spelling variants of this product

Oxygen consumption rate assay kit Extracellular Oxygen Consumption Assay Kit

4 protocols using oxygen consumption assay kit

Oxygen Consumption Assay Protocol

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Oxygen consumption was measured using an oxygen consumption assay kit (Abcam) following the manufacturer's instructions. The assay is based on the ability of oxygen to quench the excited state of oxygen consumption reagent presents in the kit, and the consumption of oxygen is reflected by an increase in phosphorescence signal. Briefly, the culture medium was added with oxygen consumption reagent and sealed with high‐sensitivity mineral oil to limit diffusion of ambient oxygen. The fluorescent signal (excitation of 380 nm/emission of 650 nm) was detected using a microplate reader (Bio‐Tek).

Wang J., Guan H., Liu H., Lei Z., Kang H., Guo Q., Dong Y., Liu H., Sun Y., Fang Z, & Li F. (2019). Inhibition of PFKFB3 suppresses osteoclastogenesis and prevents ovariectomy‐induced bone loss. Journal of Cellular and Molecular Medicine, 24(3), 2294-2307.

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Measuring Cellular Oxygen Consumption

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This assay was used to measure the oxygen consumption rate in experimental samples using an oxygen consumption assay kit from Abcam, as per the manufacturer’s instructions.

Zhai Y., Behera J., Tyagi S.C, & Tyagi N. (2019). Hydrogen sulfide attenuates homocysteine-induced osteoblast dysfunction by inhibiting mitochondrial toxicity. Journal of cellular physiology, 234(10), 18602-18614.

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Fatty Acid Oxidation Measurement in Tissues

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Liver or adipose tissues were dissected, weighted, quickly rinsed in PBS. Minced tissues were placed in 96-well tissue culture plate. For cultured cells, PBS was used to rinse the cells. Fatty Acid Oxidation was measured with combination of Fatty Acid Oxidation Assay Kit (Abcam) and Oxygen Consumption Assay Kit (Abcam) according to the manufacturer’s instruction. 150μl reaction medium and 10μl oxygen consumption reagent were added to each well. Wells were sealed with pre-warmed high sensitivity mineral oil. Fluorescence was measured at 37°C for 30min by Tecan Infinite M200 Pro.

Sun X., Seidman J.S., Zhao P., Troutman T.D., Spann N.J., Que X., Zhou F., Liao Z., Pasillas M., Yang X., Magida J.A., Kisseleva T., Brenner D.A., Downes M., Evans R.M., Saltiel A.R., Tsimikas S., Glass C.K, & Witztum J.L. (2019). Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic Steatohepatitis. Cell metabolism, 31(1), 189-206.e8.

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Adipose Tissue Oxygen Consumption Assay

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Adipose tissues were dissected, weighted, quickly rinsed in PBS. Minced tissues were placed in 96-well tissue culture plate. Ex vivo oxygen consumption rate was measured with Oxygen Consumption Assay Kit (Abcam) according to the manufacturer’s instruction. 150μl pre-warmed medium and 10μl oxygen consumption reagent were added to each well. Wells were sealed with pre-warmed high sensitivity mineral oil. Fluorescence was measured at 37°C for 30min by Tecan Infinite M200 Pro.

Zhao P., in Wong K., Sun X., Reilly S.M., Uhm M., Liao Z., Skorobogatko Y, & Saltiel A.R. (2018). TBK1 at the crossroads of inflammation and energy homeostasis in adipose tissue. Cell, 172(4), 731-743.e12.

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