Ab124928

Cells were lysed with RIPA lysis buffer to extract total protein. SDS-PAGE gels were prepared, followed by ECL chemiluminescence and exposure development. The primary antibodies (concentrations) used were anti-TLR7 (1:3000), anti-MyD88 (1:1000), anti-IRAK-1 (phospho-T209) (1:300), and phospho-NF-κBp65 antibodies (1:1000). Reagent manufacturers and lot number: PAGE Gel Rapid Preparation Kit: Yamei, PG112; anti-TLR7 antibody: abcam, ab124928; anti-MyD88 antibody: abcam, ab2064; anti-IRAK-1 (phospho-T209) antibody: abcam, ab218130; phospho-NF-κBp65 antibody (Ser536): CST, 3033S; Beta actin antibody: BIOKEN, BK7018.

The following antibodies were used in the study: rabbit anti-human TLR2 (ab213676 at dilution 1:200), mouse anti-human TLR4 (ab22048 at dilution 1:100), rabbit anti-human TLR7 (ab124928 at dilution 1:100), mouse anti-human TLR9 (ab134368 at dilution 1:200), rabbit anti-human RAGE (ab3611 at dilution 1:100), rabbit anti-human HMGB1 (ab79823 at dilution 1:400), and rabbit anti-human NF-κB p65 (ab16502 at dilution 1:1000) (all from Abcam, UK).