Il 10 jes319f1

The ELISA kit of OVA-specific IgE was purchased from AbD Serotec (Raleigh, NC); ELISA kits and proteins of IL-4, IL-5 and IL-13 were purchased from R&D Systems (Minneapolis, MN). The fluorochrome-labeled antibodies of CD19 (SJ25C1) and IL-10 (JES319F1) for flow cytometry were purchased from BD Biosciences (Franklin Lakes, NJ). The anti-IL-10 polyclonal antibody (V-15) was purchased from Santa Cruz Biotech (Santa Cruz, CA). The reagents for RT-qPCR and Western blotting were purchased from Invitrogen (Carlsbad, CA). The reagents for constructing the liposomes were purchased from Sigma Aldrich (St. Louis., MO). The reagent kits for immune cell isolation were purchased from Miltenyi Biotech (San Diego, CA).

The following antibodies were used for flowcytometry: CD3 (HIT3a, Becton-Dickinson (BD)), CD4 (SK3, BD), CD45RA (HI100, Biolegend), CXCR5 (TG2/CXCR5,Biolegend and RF8B2,BD), CCR7 (3D12, BD), PD-1 (NAT), CD25 (2A3, BD), CD127 (A7R34, eBioscience), CRTAM(R&D), Granzyme A (GB11,BD), Foxp3 (259D/C7, BD), CD57(HNK-1,BD), IL-17 (SCPL1362, BD), IL-10 (JES3-19F1,BD), IFN-g (25723.11,BD), IL-4 (7A3-3,BD), IL-21 (3A3-N2.1, BD), STAT3(4/P-STAT3,BD), and cell trace violet (CTV,Life Technologies). Data were acquired on FACSCaliber or FACSCanto II and data were then analyzed with FlowJo, version (8.7) (Treestar).