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3 protocols using 4 hydroxy 3 methoxycinnamaldehyde

1

Quantification of Phenolic Compounds

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Ethyl gallate, 4-hydroxybenzaldehyde, vanillin, tyrosol, protocatechuic aldehyde, syringaldehyde, pyrogallol, 4-hydroxy-3-methoxycinnamaldehyde, 4-methylcatechol, tryptophol, ethyl 3,4-dihydroxycinnamate, 2,6-dimethoxyphenol, ethyl vanillate, homovanillyl alcohol, eugenol, 4-hydroxy-3-methoxyphenylacetone, salicylaldehyde, coniferyl alcohol, epicatechin, catechin, scopoletin, p-coumaric acid, caffeic acid, resveratrol, syringic acid, rutin, gallic acid, salicylic acid, protocatechuic acid, 2-methoxy-4-vinylphenol, 4-vinylphenol, 4-ethylguaiacol were purchased from Sigma-Aldrich (Steinheim, Germany). All chemicals were used in the highest purity available. Acetonitrile and methanol (gradient grade for liquid chromatography) were also purchased from Sigma-Aldrich. Deionized water was produced by Mili-Q® Reference System (Merck KGaA, Darmstadt, Germany). Sodium chloride was purchased by Lach-Ner (Neratovice, Czech Republic). Standard solution of n-alkanes C8-C33 was purchased by CPAchem (Stara Zagora, Bulgaria). Technical gases, i.e., helium 4.6, hydrogen, and nitrogen, were purchased from Linde Gas (Prague, Czech Republic).
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2

Bioactive Compounds Extraction Protocol

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4-hydroxy-3-methoxycinnamaldehyde, homovanilyl alcohol, vanillin, syringic acid, vanillic acid, gallic acid, dihydroferulic acid, p-coumaric acid, hydroquinone, ferulic acid, homovanillic acid, 4-hydroxybenzoic acid and vanillylidenacetone and other reagents used for growth media preparation in the studies were procured from Sigma-Aldrich.
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3

Synthesis and Characterization of Organic Compounds

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2-propanol-d8 (99%), KBr (99.95%), (E)-benzalacetone, (E)-4-(furan-2-yl)but-3-en-2-one (98.0%), 1,3-diphenyl-propen-3-one (97%), 1-propenylbenzene (96%), 4-phenyl-1-butene were acquired from Shanghai Aladdin Biochemical Technology Co., Ltd. 2-methylimidazole (99%), 1-propanol (99.5%), (E)-3-(2-furanyl)-2-propenal (98%), cinnamyl alcohol (98%) were purchased from J&K Scientific Ltd. Methanol (99.7%), ethanol (99.7%), 2-propanol (99.8%), pyridine (99.5%), benzyl alcohol were acquired from Concord Technology (Tianjin) Co., Ltd. KSCN (99.5%), (E)-1-phenyl-1-butene (97%), triphenylmethane (99%), cinnamaldehyde (98%) were purchased from Shanghai Macklin Biochemical Co., Ltd. 1-butanol (99.5%), triphenylMethanol (99%),dodecane (99%), boric acid (99.5%), Zn(NO3)2·6H2O, propylbenzene (99%), allylbenzene (98%), chitosan (99%) were acquired from Beijing InnoChem Science & Technology Co., Ltd. Cinnamyl acetate (97%) was acquired from TCI (Shanghai) Development Co., Ltd. 3-phenyl-1-propanol (98%) was purchased from Adamas-Beta. 4-hydroxy-3-methoxycinnamaldehyde (97%) was acquired from Sigma-Aldrich. t-butanol was purchased from Alfa Aesar. Nitrogen (99.99%), argon (99.99%) and hydrogen (99.99%) were provided by Beijing Analytic Instrument Company. All reagents were directly used without further purification.
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