Anti paxillin antibody

Manufactured by Merck Group

The Anti-paxillin antibody is a laboratory tool used to detect and study the paxillin protein in biological samples. Paxillin is a focal adhesion protein involved in cell-matrix interactions and signaling. The antibody can be used in various techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to identify and analyze the presence and distribution of paxillin in cells and tissues.

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Anti-paxillin Paxillin

2 protocols using anti paxillin antibody

Antibodies and Reagents for Cell Signaling

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Anti-SCGN antibody was from AbFrontier. Anti-FAK, anti-paxillin, anti-phospho-paxillin (Tyr¹¹⁸), anti-ERK1/2, anti-phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴), anti-Akt and anti-phospho-Akt (Ser⁴⁷³) antibodies were from Cell Signaling Technology. Anti-α-tubulin antibody, anti-β-actin antibody and normal rabbit IgG were from Santa Cruz Biotechnology. Anti-phospho-FAK (Tyr³⁹⁷) and anti-SCGN antibodies used in immunoprecipitation were from Abcam. anti-paxillin antibody used in confocal microscopy was from Millipore Corporation. Anti-E-cadherin (epithelial cadherin) and anti-N-cadherin (neural cadherin) antibodies were from BD Biosciences. Horseradish peroxidase-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were from Bio-Rad Laboratories. Rhodamine–phalloidin, Alexa Fluor® 488- or Alexa Fluor® 568-conjugated goat anti-rabbit IgG and Alexa Fluor® 488-conjugated goat anti-mouse IgG were from Invitrogen. Latrunculin B was from Calbiochem. Cytochalasin D, ionomycin and DMSO from Sigma–Aldrich. Penicillin G, streptomycin, FBS and trypsin were from Gibco Life Technologies. DMEM (Dulbecco's modified Eagle's medium) and 45% D-glucose were from WelGENE. SMARTpool siRNA and DharmaFECT1 transfection reagent were from Dharmacon. Insulin ELISA kit was from ALPCO. BCA protein assay was from Thermo Scientific. Protein G–Sepharose beads and silver staining kit were from GE Healthcare.

Yang S.Y., Lee J.J., Lee J.H., Lee K., Oh S.H., Lim Y.M., Lee M.S, & Lee K.J. (2016). Secretagogin affects insulin secretion in pancreatic β-cells by regulating actin dynamics and focal adhesion. Biochemical Journal, 473(12), 1791-1803.

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Cytoskeletal Dynamics in Response to LPA

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1 × 10⁴ cells were seeded on glass coverslips in 12-well plates. After seeding, cells were serum starved for 24 hr. Cells were treated with 10μM lysophosphatidic acid for 30 minutes and then fixed with 4% paraformaldehyde in PBS and permeabilized with 0.2% Triton-X-100. F-actin was visualized by staining with TRICT conjugated phalloidin (Sigma Aldrich). Focal adhesions were stained with anti-paxillin antibody (Millipore). Nuclei were counter-stained with DAPI (Calbiochem). Immunofluorescence images were captured under Leica Q550CW fluorescence microscope (Leica) at 100× magnification. For scanning electron microscopy, cells were fixed with 2.5% glutaraldehyde and 1% osmium tetroxide. Fixed cells were then subjected to stepwise ethanol dehydration and critical point drying. Images were scanned and captured under 2,500× magnification using a Hitachi S-4800 FEG Scanning Electron Microscope (Hitachi).

Wong C.M., Wei L., Au S.L., Fan D.N., Zhou Y., Tsang F.H., Law C.T., Lee J.M., He X., Shi J., Wong C.C, & Ng I.O. (2015). MiR-200b/200c/429 subfamily negatively regulates Rho/ROCK signaling pathway to suppress hepatocellular carcinoma metastasis. Oncotarget, 6(15), 13658-13670.

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