Cg15kh

Manufactured by Thorlabs

Sourced in United States

The CG15KH is a precision laboratory optical chopper wheel from Thorlabs. It is designed to modulate continuous-wave light sources at frequencies up to 15 kHz. The device features an aluminum chopper wheel with a 15 mm aperture and is suitable for use with various Thorlabs chopper controllers.

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Lab products found in correlation

Discovermp software, by Refeyn (1 mentions) Ab7481, by Abcam (1 mentions) Ms10uw, by Thorlabs (1 mentions) Vt1200s vibratome, by Leica (1 mentions) Mpeg sil 5000, by Laysan Bio (1 mentions)

4 protocols using cg15kh

Quantitative mRNA Characterization via MP

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MP
measurements were carried out on a TwoMP
Auto instrument (Refeyn, UK) at room temperature, i.e., approximately
24 °C. High-precision microscope cover glasses, 24 mm ×
50 mm, Thorlabs CG15KH were used and coated with poly-l-lysine
(PLL). PLL is positively charged and is used to increase the amount
of binding events of negatively charged nucleic acids, which greatly
increases detection. Samples were diluted in a PBS buffer and a final
mRNA amount of 50 ng in droplets was analyzed. For each experiment,
thousands of mRNA molecules were counted and their contrasts were
measured. Standardized ssRNA ladder (New England Biolabs, cat# N0364)
calibration was used to determine the number of nucleotides. Movie
acquisition was performed for 60 s with the DiscoverMP software (version
2022 R1, Refeyn Ltd.), and the data were analyzed using the range
tool parameters for more accurate mass and purities. Data was analyzed
using DiscoverMP v2.0.

Camperi J., Lippold S., Ayalew L., Roper B., Shao S., Freund E., Nissenbaum A., Galan C., Cao Q., Yang F., Yu C, & Guilbaud A. (2024). Comprehensive Impurity Profiling of mRNA: Evaluating Current Technologies and Advanced Analytical Techniques. Analytical Chemistry, 96(9), 3886-3897.

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Immunofluorescence Imaging of Lphn2 in Mouse Brain

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For Lphn2 and synaptic immunofluorescence, Lphn2^mVenus transgenic mice were perfused transcardially with 20 mL PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 1.8 mM KH₂PO₄, pH 7.4) and 10 mL fresh 4% paraformaldehyde (PFA) in PBS. Brains were dissected out, placed in 4% PFA at 4°C overnight, briefly rinsed in PBS, and mounted in agarose. Horizontal sections (100 μm) were prepared using a VT1200S vibratome (Leica). Dorsal hippocampal sections were washed for 5 min in PBS and blocked for 1 h at room temperature with 10% normal goat serum (NGS; ab7481, Abcam) and 0.5% Triton X-100 in PBS. Subsequently, slices were incubated overnight at 4°C in PBS containing 1% NGS, 0.01% Triton, and primary antibody. Sections were washed with PBS 3 times for 5 min each and then incubated in PBS containing 1% NGS, 0.01% Triton, and secondary antibody for 4 h at room temperature. Sections were washed again with PBS (3 washes, 5 min each) and then mounted on glass slides (MS10UW, Thorlabs) using Vectashield Plus Antifade mountant (Vector Laboratories, H-1900-10) and #1.5H high-precision cover glass (CG15KH, Thorlabs).

Murphy T.R., Amidon R.F., Donohue J.D., Li L, & Anderson G.R. (2024). Synaptic cell-adhesion molecule latrophilin-2 is differentially directed to dendritic domains of hippocampal neurons. iScience, 27(2), 108799.

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Functionalization of Glass Coverslips

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Coverslips (Thorlabs, CG15KH) were placed into a glass jar, bathed in acetone for 30 min, incubated in ethanol for 15 min, and washed in MiliQ water three times. Subsequently, the coverslips were sonicated in 2% Hellmanex or 2% Micro90 for 2 h at room temperature in a water bath sonicator, followed by series of washes in MiliQ water. Coverslips were then transferred into a 0.1 M KOH bath, incubated for 30 min, washed in MiliQ water and dried with clean nitrogen gas. For functionalization, coverslips were submerged in a glass jar containing mPEG-Silane (Laysan Bio Inc, MPEG-SIL-5000) or Biotin-mPEG-Silane (Laysan Bio Inc, Biotin-PEG-SIL-5K) solution, and protected from light for 18 h. The next day, coverslips were washed with clean ethanol and MiliQ water. Finally, coverslips were dried once again with clean nitrogen gas and stored at 4 °C for up to 2 weeks.

Morales E.A., Fitz G.N, & Tyska M.J. (2024). Mitotic spindle positioning protein (MISP) preferentially binds to aged F-actin. The Journal of Biological Chemistry, 300(5), 107279.

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Precise Micro-Syringe Printing of Quantum Dots

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The flow rate and flow duration of the micro-syringe pumps are adjusted to generate the above-mentioned compositions by precise mixing of QD inks.
A custom Python code is adapted [54 (link)] to control both the pumps and the 3-axis micro-positioning gantry (Aerotech, PA, USA). RS-232-to-USB connectors are used to interface the microsyringe pumps with the PC and the gantry system is interfaced using a PCIe FireWire adapter. Serial communication protocol is used to control the hardware from the PC. The Python code controls the flow duration and flow rate while the print is in motion, providing comprehensive control of the printing parameters. Assisted by the 3-axis micro-positioning gantry with a custom G-code, QD droplets are printed using a 25-gauge needle (GA) at a constant height of 80 µm from the substrate. For substrate, we used 170±5 µm thick glass slides (CG15KH, Thorlabs, NJ, USA) cleaned using acetone and isopropyl alcohol and dried using compressed nitrogen gas. The printed droplets are evaporated at ambient temperature and humidity (21 °C ± 1°C and 30±2 RH%) prior to the imaging.

Ghosh S., Johnson M.V., Neupane R., Hardin J., Berrigan J.D., Kalidindi S.R, & Kong Y.L. (2022). Machine learning-enabled feature classification of evaporation-driven multi-scale 3D printing. Flexible and printed electronics, 7(1), 014011.

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