Frt19a

Manufactured by BD

The FRT19A is a laboratory equipment product designed for specific technical applications. It serves a core function within its intended use, but detailed information about its features and capabilities is not available for this unbiased, factual description.

Automatically generated - may contain errors

Lab products found in correlation

Uas mcd8 gfp, by BD (1 mentions) Elav gal4, by BD (1 mentions) Uas dcr2, by BD (1 mentions) Repo gal4, by BD (1 mentions) Frt40a, by BD (1 mentions) W1118 flies, by BestGene (1 mentions)

3 protocols using frt19a

Comprehensive Drosophila Genetics Toolkit

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

FRT19A, fzr^A (#52384), FRT19A, fzr^B (#52385), and Dp(1;3)DC120 (#30265) were obtained from BDSC. FRT19A, fzr^G0418 (#111943) was obtained from Kyoto Stock Center. UAS-Fzr-HA (#F000893) was obtained from FlyORF. UAS-HA-Rca1 is a gift from Dr. Frank Sprenger (Grosskortenhaus and Sprenger, 2002 (link)) and FRT19A, fzr^8F3 (P{neoFRT}19A/FM7) was a gift from Dr. Christian Klambt. EGFP-Fzr^BAC was generated in this study.
RNAi lines used in this study: erm_RNAi (BDSC #26778), fzr_RNAi (GD#25553), cdc20_RNAi_1 (GD#40500), cdc20_RNAi_2 (GD#44834), ida_RNAi (BDSC#34522); brm_RNAi (GD#37720), β-gal_RNAi (BDSC#50680), and bcd_RNAi (GD#48966).
Neural stem cell drivers included insc-Gal4 (BDSC#8751; 1407-Gal4) or wor-Gal4 (BDSC#56553). Glial driver was repo-Gal4 (BDSC#7415). Type I NSC driver (ase-Gal4; UAS-mCD8-GFP, T. Lee). Type II NSC driver (w; UAS-Dicer2, wor-Gal4, ase-Gal80; UAS-mCD8-GFP) (Bowman et al., 2008 (link)). INP driver (erm-Gal4/CyO) (Pfeiffer et al., 2008 (link)). Other drivers used in this study are: nerfin-1-Gal4, UAS-mCD8-GFP (Louis Y. Cheng), pros-Gal4 (BDSC#80572), and elav-Gal4 (BDSC#458). UAS-Dcr2 (BDSC#24650) or/and UAS-CD8-GFP (BDSC#32186) was used together with various Gal4 drivers or RNAi stocks.
Experiments with mutants were performed at 25°C, and experiments for RNAi-mediated knockdown or overexpression were carried out at 29°C.

Ly P.T, & Wang H. (2020). Fzr/Cdh1 Promotes the Differentiation of Neural Stem Cell Lineages in Drosophila. Frontiers in Cell and Developmental Biology, 8, 60.

+ Open protocol

+ Expand

Transgenic and Mutant Fly Stocks for Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following transgenic and mutant fly stocks were used: ft^fd (Bryant et al., 1988 (link)), E(spl)mδ0.5 (Cooper and Bray, 1999 (link)), numb¹⁵ (Berdnik et al., 2002 (link)), numb¹ (BDSC 4096), numb² (Uemura et al., 1989 (link)), numb4 (Skeath and Doe, 1998 (link)), P(PZ)numb⁰³²³⁵ (BDSC 11278), P(EPgy2)numb^EY03840, FRT40A (Kyoto 114573), sev-GAL4 (Richardson et al., 1995 (link)), UAS-Numb (Frise et al., 1996 (link)), UAS-Numb-GFP (Song and Lu, 2012 (link)), UAS-CD8-GFP (BDSC 5136), dsh³, FRT19A (BDSC 6331), dsh¹ (BDSC 5298), lgl⁴ (Gateff and Schneiderman, 1974 (link)), lgl^4w3 (Bilder et al., 2000 (link)), tubGAL80, FRT40A (BDSC 5192), Diap1-lacZ (P(lacW)th^j5C8 (Ryoo et al., 2002 (link))).
Clones of mutant eye tissue were generated by the Flp/FRT technique (Golic, 1991 (link)). Flipase expression was induced under the control of the eyeless promoter (Newsome et al., 2000 (link)).

Domingos P.M., Jenny A., Combie K.F., del Alamo D., Mlodzik M., Steller H, & Mollereau B. (2019). Regulation of Numb during planar cell polarity establishment in the Drosophila eye. Mechanisms of development, 160, 103583.

+ Open protocol

+ Expand

Drosophila Neuronal Nemp Gene Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

WT flies are yw unless otherwise noted. RNA interference (RNAi) stocks for expression of double-stranded RNA under control of GAL4/UAS were obtained from Vienna Drosophila Resource Center (VDRC) or Bloomington Drosophila Stock Center (BDSC) (dNemp VDRC #37412, dNemp BDSC #57475 for germline expression) and were driven using enGal4, nosGal4, or tubGal4. dNemp^−/− clones were generated with FRT19A (BDSC #1744). BAC rescue constructs were generated using flies containing an attP2 site on 3L (BDSC #8622). UAS-dNemp and UAS-hNEMP1 were generated by injecting w[1118] flies (BestGene Inc) with cDNA dNemp1 or hNEMP1 pPWH (Gateway), respectively, and driven using tubGal4 in a dNemp^−/− background.

Tsatskis Y., Rosenfeld R., Pearson J.D., Boswell C., Qu Y., Kim K., Fabian L., Mohammad A., Wang X., Robson M.I., Krchma K., Wu J., Gonçalves J., Hodzic D., Wu S., Potter D., Pelletier L., Dunham W.H., Gingras A.C., Sun Y., Meng J., Godt D., Schedl T., Ciruna B., Choi K., Perry J.R., Bremner R., Schirmer E.C., Brill J.A., Jurisicova A, & McNeill H. (2020). The NEMP family supports metazoan fertility and nuclear envelope stiffness. Science Advances, 6(35), eabb4591.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!