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V2 300 cycles miseq reagent kit

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The V2 300-cycles Miseq reagent kit is a laboratory equipment product designed for use with the Illumina MiSeq system. The kit provides the necessary reagents for performing up to 300 sequencing cycles on the MiSeq platform.

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Lab products found in correlation

Hotstar taq plus kit, by Qiagen (2 mentions) Miseq instrument, by Illumina (2 mentions)

Spelling variants of this product

MiSeq Reagent Kit (196 citations) MiSeq v2 (90 citations) MiSeq v2 kit (38 citations) Reagent Kit v2 (26 citations) MiSeq 300 (25 citations) MiSeq V2 300 cycle (9 citations) 300-cycle v2 kit (9 citations) 300-cycle reagent kit (4 citations) Illumina MiSeq reagents (4 citations) MiSeq kits (2 citations)

2 protocols using v2 300 cycles miseq reagent kit

1

Targeted Genetic Variant Screening

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Universal adapter sequences were tagged to the 5’ and 3’ end of target-specific primers of approximately 200±20bp in length. Based on our initial experiments that showed clustering of variants within specific exons of the 3 genes, subsequent analyses were restricted to RRAGC (exons 1 and 2), ATP6V1B2 (exons 11 and 12) and ATP6AP1 (exons 9 and 10). Primer sequences are shown in Supplementary Table 13. 100ng of genomic DNA were amplified in 2 to 4-plex PCR reactions using non-overlapping tagged-primers with the HotStar Taq Plus kit (Qiagen) under limited cycling conditions. Amplified PCR fragments were subsequently pooled in equimolar ratios by sample and prepared for sequencing with the attachment sample specific indexes and Illumina adaptor sequences. Indexed libraries were pooled and sequenced on a single lane of an Illumina MiSeq instrument using the V2 300-cycles Miseq reagent kit (Illumina) generating 150-bp paired end reads. Each sample was screened in duplicate. Variant calling and annotation are as described above.
Okosun J., Wolfson R.L., Wang J., Araf S., Wilkins L., Castellano B.M., Escudero-Ibarz L., Al Seraihi A.F., Richter J., Bernhart S.H., Efeyan A., Iqbal S., Matthews J., Clear A., Guerra-Assunção J.A., Bödör C., Quentmeier H., Mansbridge C., Johnson P., Davies A., Strefford J.C., Packham G., Barrans S., Jack A., Du M.Q., Calaminici M., Lister T.A., Auer R., Montoto S., Gribben J.G., Siebert R., Chelala C., Zoncu R., Sabatini D.M, & Fitzgibbon J. (2015). Recurrent mTORC1-activating RRAGC mutations in follicular lymphoma. Nature genetics, 48(2), 183-188.
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2

Targeted Sequencing of RRAGC, ATP6V1B2, and ATP6AP1

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Universal adapter sequences were tagged to the 5′ and 3′ end of target-specific primers of approximately 200±20bp in length. Based on our initial experiments that showed clustering of variants within specific exons of the 3 genes, subsequent analyses were restricted to RRAGC (exons 1 and 2), ATP6V1B2 (exons 11 and 12) and ATP6AP1 (exons 9 and 10). Primer sequences are shown in Supplementary Table 13. 100ng of genomic DNA were amplified in 2 to 4-plex PCR reactions using non-overlapping tagged-primers with the HotStar Taq Plus kit (Qiagen) under limited cycling conditions. Amplified PCR fragments were subsequently pooled in equimolar ratios by sample and prepared for sequencing with the attachment sample specific indexes and Illumina adaptor sequences. Indexed libraries were pooled and sequenced on a single lane of an Illumina MiSeq instrument using the V2 300-cycles Miseq reagent kit (Illumina) generating 150-bp paired end reads. Each sample was screened in duplicate. Variant calling and annotation are as described above.
Okosun J., Wolfson R.L., Wang J., Araf S., Wilkins L., Castellano B.M., Escudero-Ibarz L., Al Seraihi A.F., Richter J., Bernhart S.H., Efeyan A., Iqbal S., Matthews J., Clear A., Guerra-Assunção J.A., Bödör C., Quentmeier H., Mansbridge C., Johnson P., Davies A., Strefford J.C., Packham G., Barrans S., Jack A., Du M.Q., Calaminici M., Lister T.A., Auer R., Montoto S., Gribben J.G., Siebert R., Chelala C., Zoncu R., Sabatini D.M, & Fitzgibbon J. (2015). Recurrent mTORC1-activating RRAGC mutations in follicular lymphoma. Nature genetics, 48(2), 183-188.
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