Salmon sperm dna

Manufactured by GE Healthcare

Salmon sperm DNA is a laboratory product derived from the DNA extracted from salmon sperm. It is a common biomaterial used in various scientific applications, including cell culture, molecular biology, and genetic research. The core function of salmon sperm DNA is to provide a source of high-quality, double-stranded DNA for experimental purposes.

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Lab products found in correlation

Dynabeads m 280 streptavidin, by Thermo Fisher Scientific (2 mentions) Poly di dc, by Roche (1 mentions)

2 protocols using salmon sperm dna

Quantifying Protein-DNA Interactions

Cited 1 time

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Immobilized template assays were performed as described (Chen et al. 2009 (link)). A mouse Ucp1 enhancer fragment was prepared by PCR using biotinylated oligonucleotides and immobilized on streptavidin-conjugated magnetic beads (Dynabeads M280 streptavidin, Invitrogen). After incubating the beads in blocking buffer (50 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.01% NP-40, 1 mg/mL BSA, 0.5 mM PMSF, 10 mM DDT, 10 μg/mL salmon sperm DNA [GE Healthcare], 10 μg/mL poly dI–dC [Roche]), purified proteins were added to the reaction and incubated for 30 min at room temperature. The beads were then washed with wash buffer (50 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.01% NP-40, 0.5 mM PMSF, 0.5 mM DTT) three times, and the bound proteins were eluted by boiling in 1× Laemmli sample buffer and analyzed by immunoblot. The standard 100-μL reaction contained 5 μL of beads, 400 ng of DNA fragment, 40 ng of TRα, 40 ng of RXRα, 40 ng of PPARγ, 400 ng of MED1, 400 ng of PGC-1α, and 400 ng of PRDM16.

Iida S., Chen W., Nakadai T., Ohkuma Y, & Roeder R.G. (2015). PRDM16 enhances nuclear receptor-dependent transcription of the brown fat-specific Ucp1 gene through interactions with Mediator subunit MED1. Genes & Development, 29(3), 308-321.

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Immobilized Template Assay for BCL6

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Immobilized template assays were performed as described (Chen et al. 2009 (link)). A human BCL6 CE1 enhancer fragment, its deletion series, or OCTA mutant was prepared by PCR using biotinylated oligonucleotides (Figure S3B and Table S6) and immobilized on streptavidin-conjugated magnetic beads (Dynabeads M280 streptavidin, Invitrogen). After incubating the beads in blocking buffer {50 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.01 % NP-40, 1 mg/mL BSA, 0.5 mM PMSF, 10 mM DDT, 10 μg/mL salmon sperm DNA [27-4565-01, GE Healthcare], 10 μg/mL poly-deoxy-inosinic-deoxy-cytidylic acid [poly [d(I–C)], 10108812001, Millipore Sigma]}, purified proteins were added to the reaction and incubated for 30 min at room temperature. The beads were then washed with wash buffer (50 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.01 % NP-40, 0.5 mM PMSF, 0.5 mM DTT) three times, and the bound proteins were eluted by boiling in 100D7 Laemmli sample buffer, separated by SDS-PAGE, and analyzed by immunoblot using indicated antibodies.

Chu C.S., Hellmuth J.C., Singh R., Ying H.Y., Skrabanek L., Teater M.R., Doane A.S., Elemento O., Melnick A.M, & Roeder R.G. (2020). Unique immune cell coactivators specify locus control region function and cell stage. Molecular cell, 80(5), 845-861.e10.

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