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4600 spectrofluorometer

Manufactured by Hitachi
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The 4600 spectrofluorometer is a laboratory instrument designed for measuring the fluorescence properties of samples. It is capable of recording emission and excitation spectra.

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Lab products found in correlation

U 2910 spectrophotometer, by Hitachi (2 mentions) Fluoview fv1000 laser scanning microscope, by Olympus (1 mentions) Av 400 instrument, by Brucker (1 mentions) Tsq quantum access agilent 1100, by Thermo Fisher Scientific (1 mentions)

2 protocols using 4600 spectrofluorometer

1

NMR, MS, and Fluorescence Analysis

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All reagents and solvents are of analytical grade and used without further purification.
Nuclear magnetic resonance (NMR) spectra were measured with a Brucker AV 400 instrument, and chemical shifts were given in ppm from tetramethylsilane (TMS). Mass spectra (MS) were recorded on a Thermo TSQ Quantum Access Agilent 1100. Fluorescence emission spectra were conducted on a Hitachi 4600 spectrofluorometer. UV-Vis spectra were obtained on a Hitachi U-2910 spectrophotometer. Fluorescence imaging was performed by confocal fluorescence microscopy on an Olympus FluoView Fv1000 laser scanning microscope. pH was conducted with a pH-meter PBS-3C.
Yu C., Wen Y, & Zhang J. (2014). Synthesis of a Cu2+-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging. Sensors (Basel, Switzerland), 14(11), 21375-21384.
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2

Characterization of Metal Ion Complexes

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All reagents and solvents are of analytical grade and used without further purification. The metal ions salts employed were NaCl, KCl, CaCl2·2H2O, MgCl2·6H2O, Zn(NO3)2·6H2O, PbCl2, FeCl3·6H2O, CrCl3·6H2O, CoCl2·6H2O, NiCl2·6H2O, CuCl2·2H2O, CdCl2, Hg(NO3)2, AgNO3 and AlCl3·6H2O, respectively.
Fluorescence emission spectra were conducted on a Hitachi 4600 spectrofluorometer. UV-Vis spectra were obtained on a Hitachi U-2910 spectrophotometer. Nuclear magnetic resonance (NMR) spectra were measured with a Brucker AV 400 instrument and chemical shift were given in ppm from tetramethylsilane (TMS). Mass (MS) spectra were recorded on a Thermo TSQ Quantum Access Agilent 1100.
Yu C., Jian L., Ji Y, & Zhang J. (2018). Al(iii)-responsive “off–on” chemosensor based on rhodamine derivative and its application in cell imaging. RSC Advances, 8(54), 31106-31112.
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