Wb 800s plus

Hydration Status. For assessment of hydration status, each 24 h urine sample was measured for urine volume (U_VOL) using a digital scale to the nearest 0.0001 kg (Ranger 3000, OHAUS Corporation, Parsippany, NY), urine osmolality (U_OSM) performed in duplicate using freezing point depression (Model 3320, Advanced Instruments, Inc., Norwood, MA), urine specific gravity (U_SG) (Reichert AR200, Reichert Technologies, Buffalo, NY), and urine color (U_COL) [29 (link)]. For the purposes of this manuscript, euhydration was defined as a U_OSM < 500 mOsm∙kg⁻¹ [23 (link)], U_SG ≤ 1.012, and U_COL ≤ 4 [30 (link)].
Body Composition. Nude body mass was measured to the nearest 0.1 kg using a digital scale (WB-800S Plus, Tanita Corporation, Tokyo, Japan) and height was measured to the nearest 0.1 cm using a wall-mounted stadiometer (Model 216, Seca, Chino, CA, USA). Body density was measured using the methods established by Jackson and Pollock [28 (link),29 (link)]. The average of two skinfold measures (Lange Skinfold Calipers, Beta Technologies, Santa Cruz, CA) taken across three sites on the right side of the body were used; measures were taken at the chest, abdomen and thigh for men [31 (link)] and suprailiac, triceps, and thigh for women [32 (link)]. Body fat percentage was estimated using the method established by Siri [33 ].

Anthropometric measures of height and weight were taken using a Seca stadiometer (Model 216, Chino, CA, USA) and Tanita scale (WB-800S Plus, Tanita Corporation, Tokyo, Japan), respectively. Height (m) and weight (kg) measurements were used to calculate a participant’s BMI (kg/m²). Age and race were self-reported as part of the demographic questionnaire during the introductory appointment.

Height was measured to the nearest 0.1 cm using a wall-mounted stadiometer (Model216; Seca, Chino, CA, USA) and nude body mass (NBM) was measured to the nearest 0.1 kg using a digital scale (WB-800S Plus; Tanita Corporation, Tokyo, Japan). For each experimental trial, body water composition (e.g., intra- and extracellular fluid) was measured immediately following fluid consumption (0 min), 30 min post ingestion, and every 60 min via bioelectrical impedance analysis (Quantum IV; RJL Systems, Clinton Township, MI, USA) [33 (link)]. Prior to the assessment, participants rested in a supine position for a minimum of 6 min to allow for equilibration of body water compartments (4). Subsequently, electrodes were placed on the ankle, wrist, lower leg, and forearm in order to assess impedance and resistance at 50 KHz at each timepoint. The values for impedance and resistance were further input into the Quantum IV software to calculate intercellular (ICF) and extracellular fluid (ECF). Bioelectrical impendence and the Quantum IV software were also used to assess body fat percentage (BF%) used for subject demographics.