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C met sirna

Manufactured by Horizon Discovery
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C-Met siRNA is a small interfering RNA (siRNA) targeting the c-Met gene, which encodes the receptor tyrosine kinase c-Met. C-Met siRNA can be used to silence the expression of the c-Met gene, which is involved in various cellular processes, including cell proliferation, migration, and survival.

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Lab products found in correlation

Silectfect, by Bio-Rad (1 mentions) Akt sirna, by Horizon Discovery (1 mentions) Mir 23b 3p mimic, by GenePharma (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)

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C-siRNA SiRNAs

2 protocols using c met sirna

1

Targeted Gene Silencing in Cell Lines

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Cells were seeded in six-well plates 24 h before transfection. 20 nM Dicer siRNA (Dharmacon, Catalog No. D-003483), Drosha siRNA (Dharmacon, Catalog No. D-016996), Exportin-5 siRNA (Dharmacon, Catalog No. D-014000), Akt siRNA (Dharmacon, Catalog No. D-003000), or c-Met siRNA (Dharmacon, Catalog No. L-003156) was transfected with 1.5 μl siLectFect (Bio-Rad) for 24 h. Similar protocol was used for transfecting miR-199a-3p mimic (Ambion, Catalog No. 4464066) at 10 nM concentration. Cells were at 80% confluence at the time of transfection. Respective non-specific controls were incorporated in each experimental setup.
Hassan A.A., Artemenko M., Tang M.K., Shi Z., Chen L.Y., Lai H.C., Yang Z., Shum H.C, & Wong A.S. (2022). Ascitic fluid shear stress in concert with hepatocyte growth factor drive stemness and chemoresistance of ovarian cancer cells via the c-Met-PI3K/Akt-miR-199a-3p signaling pathway. Cell Death & Disease, 13(6), 537.
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2

miR-23b-3p Mimics and Inhibitors

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The miR-23b-3p mimic, inhibitor, and corresponding controls were purchased from GenePharma (Shanghai, China). c-MET siRNA and negative control (NC) were purchased from Dharmacon (Lafayette, CO, USA). SNU-354 and SNU-368 cells were transiently transfected with Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the instructions provided by the manufacturer. After transfection for 48 h, the cells were harvested, and the transfection efficiency was analyzed by qRT-PCR.
Park N.R., Cha J.H., Sung P.S., Jang J.W., Choi J.Y., Yoon S.K, & Bae S.H. (2022). MiR-23b-3p suppresses epithelial-mesenchymal transition, migration, and invasion of hepatocellular carcinoma cells by targeting c-MET. Heliyon, 8(10), e11135.
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