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Isolectin gs ib4 alexa 568

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Isolectin GS-IB4-Alexa 568 is a fluorescently labeled lectin derived from the seeds of the Griffonia simplicifolia plant. It is commonly used as a marker for endothelial cells and vasculature in various research applications.

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Lab products found in correlation

Anti tomm20, by Abcam (2 mentions) Isolectin gs ib4 alexa 647, by Thermo Fisher Scientific (2 mentions) Anti ng2 chondroitin sulfate proteoglycan, by Merck Group (2 mentions) Anti collagen 4, by Southern Biotech (2 mentions) Isolectin gs ib4 alexa 488, by Thermo Fisher Scientific (2 mentions) Anti cpt1a, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Alexa 568 (337 citations) Isolectin GS-IB4 (51 citations) GS-IB4 (9 citations) Isolectin GS-IB4 Alexa Fluor 568 conjugate (2 citations) Alexa Fluor 568-conjugated isolectin GS-IB4 (2 citations)

2 protocols using isolectin gs ib4 alexa 568

1

Immunofluorescence Staining of Fixed Cells

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PFA fixed cell cultures (4% PFA except for CPT1a staining where 2% PFA was used), spheroids (4% PFA) or whole mount retinas (4% PFA) were subjected to immunofluorescence staining using the following isolectin conjugates or primary antibodies: isolectin GS-IB4-Alexa 488, isolectin GS-IB4-Alexa 568, isolectin GS-IB4-Alexa 647 (Molecular Probes), anti-CPT1a (Cell Signaling), anti-NG2 Chondroitin Sulfate Proteoglycan (Chemicon), anti-Tomm20 (Abcam) and anti-collagen IV (Southern Biotech). Alexa-488, -568 or -633 conjugated secondary antibodies were used (Molecular Probes). EdU, EU and HPG staining was performed using a Click-IT assay with Alexa fluor 555 according to manufacturer’s instructions.
Schoors S., Bruning U., Missiaen R., Queiroz K.C., Borgers G., Elia I., Zecchin A., Cantelmo A.R., Christen S., Goveia J., Heggermont W., Goddé L., Vinckier S., Van Veldhoven P.P., Eelen G., Schoonjans L., Gerhardt H., Dewerchin M., Baes M., De Bock K., Ghesquière B., Lunt S.Y., Fendt S.M, & Carmeliet P. (2015). Fatty acid carbon is essential for dNTP synthesis in endothelial cells. Nature, 520(7546), 192-197.
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2

Immunofluorescence Staining of Fixed Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PFA fixed cell cultures (4% PFA except for CPT1a staining where 2% PFA was used), spheroids (4% PFA) or whole mount retinas (4% PFA) were subjected to immunofluorescence staining using the following isolectin conjugates or primary antibodies: isolectin GS-IB4-Alexa 488, isolectin GS-IB4-Alexa 568, isolectin GS-IB4-Alexa 647 (Molecular Probes), anti-CPT1a (Cell Signaling), anti-NG2 Chondroitin Sulfate Proteoglycan (Chemicon), anti-Tomm20 (Abcam) and anti-collagen IV (Southern Biotech). Alexa-488, -568 or -633 conjugated secondary antibodies were used (Molecular Probes). EdU, EU and HPG staining was performed using a Click-IT assay with Alexa fluor 555 according to manufacturer’s instructions.
Schoors S., Bruning U., Missiaen R., Queiroz K.C., Borgers G., Elia I., Zecchin A., Cantelmo A.R., Christen S., Goveia J., Heggermont W., Goddé L., Vinckier S., Van Veldhoven P.P., Eelen G., Schoonjans L., Gerhardt H., Dewerchin M., Baes M., De Bock K., Ghesquière B., Lunt S.Y., Fendt S.M, & Carmeliet P. (2015). Fatty acid carbon is essential for dNTP synthesis in endothelial cells. Nature, 520(7546), 192-197.
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