Quantikine high sensitivity human il 6 elisa kits

Four blood samples of 6mL were collected at baseline, and 30, 60, and 90 minutes after TSST onset into EDTA lavender-top tubes. Samples were placed on ice immediately after collection, after which they were centrifuged, aliquoted into plasma samples, and stored at −80°C. After data collection was completed, samples were assayed in duplicate for interleukin (IL)-6 using the Quantikine high sensitivity human IL-6 ELISA kits (R&D Systems, Inc., Minneapolis, MN) by the UCLA Cousins Center Inflammatory Biology Core. Intra- and inter-assay coefficients of variability were below 7%. Outlier screening revealed a total of 11 samples across time points that had IL-6 values greater than 3 SDs from their respective means. These values were Winsorized and replaced with the value at three SDs. All values were natural log-transformed given the skewed distribution. As reported previously (Chiang et al., 2017 (link)), the TSST was effective in eliciting an inflammatory response, with participants exhibiting a 34% average increase in IL-6 from baseline to 90 minutes after TSST onset.

Four blood samples were collected throughout the laboratory visit at baseline, and 30, 60, and 90 min after TSST onset. At each time point, six mL of blood was collected into EDTA lavender-top tubes, placed on ice immediately after collection, centrifuged for acquisition of plasma, separated into three plasma aliquots, and stored at −80 °C. Upon data collection completion, samples were assayed for IL-6. We focused on IL-6 because it increases in response to acute stress and has been associated with early adversity and long-term chronic health conditions during adulthood (Carpenter et al., 2010 (link); Slopen et al., 2013 (link); Steptoe et al., 2007 (link); Yudkin et al., 2000 (link)).
Samples were assayed in duplicate using the Quantikine high sensitivity human IL-6 ELISA kits (R&D Systems, Inc., Minneapolis, MN) by the UCLA Inflammatory Biology Core Laboratory. Each subject’s samples were assayed in the same run, and intra- and inter-assay coefficients of variability were below 7%. No sample was below the lower limit detection of .2 pg/mL. Consistent with past research (Carpenter et al., 2010 (link); Carroll et al., 2011 (link); Pace et al., 2006 (link); Slavich et al., 2010 (link)), change scores were calculated from baseline to peak IL-6 (i.e., 90 min post-stressor) to index absolute magnitude of IL-6 increases in response to the TSST.