Polyethersulfone (PES, MW 58 kDa) and polyvinylpyrrolidone (PVP, MW 360 kDa) were purchased from Basf, Ludwigshafen am Rhein, Germany and Sigma-Aldrich, St. Louis, MI, USA, respectively. The common solvent for both polymers was N, N-dimethylacetamide (DMAc, 99.5%), purchased from Tedia, Fairfield, Ohio, EUA. For microbiological experiments, deionized water was supplied by a Milli-Q apparatus (Merck KGaA, Darmstadt, Germany). Yeast extract, meat peptone, and agar were purchased from Kasvi, São José do Pinhais, Brazil. Magnesium sulfate, potassium phosphate monobasic, potassium phosphate dibasic, and glycerol were purchased from Vetec, Duque de Caxias, Brazil. These reagents were used as culture medium. PES and PVP were dried at 60 °C overnight before being used and the other reagents were used as received.
Agar
Manufactured by Kasvi
Sourced in Brazil
Agar is a laboratory culture medium used for the growth and cultivation of microorganisms. It is a gelling agent derived from red algae, providing a solid or semi-solid matrix for the growth of bacterial, fungal, and other microbial cultures. Agar is commonly used in microbiology, biotechnology, and other scientific research applications.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylpyrrolidone, by BASF (1 mentions)
Yeast extract, by Kasvi (1 mentions)
Potassium phosphate dibasic, by Vetec (1 mentions)
Glycerol, by Vetec (1 mentions)
Milli q apparatus, by Merck Group (1 mentions)
Polyethersulfone, by BASF (1 mentions)
Magnesium sulfate, by Vetec (1 mentions)
Sucrose, by Kasvi (1 mentions)
2 protocols using agar
1
Fabrication and Characterization of PES-PVP Membranes
2
Optimizing Tissue Culture Conditions for Plant Growth
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Three different photoperiods and two formulations of Murashige and Skoog culture medium (Murashige and Skoog, 1962) were used (Table 1). All treatments were supplemented with 30 g.L -1 sucrose and 6.5 g.L -1 agar (Kasvi ® Paraná, Brazil), and the pH was adjusted to 5.8. These treatments were designed based on a previous study (Trettel et al., 2018a) . After preparation of the medium, glass vials containing culture medium were autoclaved at 121 °C for 20 min and were then used for seed inoculation which was performed in an aseptic chamber. Four seeds were placed in one flask containing 50 mL of the culture medium. The flasks were then closed using clear plastic lids and were sealed using PVC film. The flasks were maintained for 80 days at the biochemical oxygen demand (BOD) at 25 °C and under three different photoperiods: 24 h, 12 h, or 16 h. The BOD was equipped with white fluorescent 30-W lamps 84.60 μmol m -2
•s -1 (Philips ® Amsterdam, Netherlands).
•s -1 (Philips ® Amsterdam, Netherlands).
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