A direct heat CO2 incubator (Forma™ 311), Varioskan® Flash Microplate Multimode Readers, and NanoDrop One UV-Vis spectrophotometer were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A LightCycler PCP was purchased from Roche (CA, USA). A Centrifuge 5417R was obtained from Eppendorf (Hamburg, Germany). Agilent 1260 HPLC/6520 QTOF-MS instrument, Eclipse Plus C18 chromatography column, and Cary Eclipse fluorescence spectrophotometer were obtained from Agilent Technologies, Inc. (Agilent, Milford, MA, USA). Ultra-High-Performance Liquid Chromatography System and ACQUITY UPLC HSS C18 column (100 × 2.1 mm, 1.7 μm) were from Waters (Milford, MA, USA). The X500 QTOF Mass spectrometer system was purchased from AB Sciex Pte. Ltd. (MA, USA).
Varioskan flash microplate multimode readers
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Varioskan® Flash Microplate Multimode Readers is a laboratory instrument designed for the detection and analysis of various samples in microplates. It is capable of performing multiple detection modes, including absorbance, fluorescence, and luminescence measurements.
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Lab products found in correlation
Agilent 1260 hplc 6520 qtof ms instrument, by Agilent Technologies (1 mentions)
Acquity uplc hss c18 column, by Waters Corporation (1 mentions)
Ultra high performance liquid chromatography system, by Waters Corporation (1 mentions)
Nanodrop one uv vis spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Centrifuge 5417r, by Eppendorf (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)
2 protocols using varioskan flash microplate multimode readers
1
Analytical Instrumentation for Metabolomics
2
Quantifying Intracellular ATP by CellTiter-Glo Assay
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Intracellular ATP was quantified using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega Corporation, Madison, WI, USA). Cell suspension (100 μL) was transferred to the wells of a white 96-well microplate (Nunc, Roskilde, Denmark) and CellTiter-Glo reagent (100 μL) was added. The plate was shaken using a plate shaker for 10 min, and luminescence was measured using Varioskan Flash Microplate Multimode Readers (Thermo Fisher Scientific, Waltham, MA, USA). Cell viability was calculated using the ratio of the luminescence of treated cells to that of control cells, and the data were expressed as percentages.
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