DPPH (2,2-diphenyl-1-picrylhydrazyl), riboflavin, and ascorbic acid were purchased from Sigma-Aldrich, USA, whereas hydrogen peroxide was obtained from HiMedia Lab Ltd., Mumbai, India. DH2O (distilled deionized water) was prepared by Camco Industries, Chandigarh (India). All other chemicals like methanol, sulphuric acid, Whatman filter paper, petroleum ether, and ethanol were purchased from HiMedia Lab Ltd., Mumbai, India.
Petroleum ether
Manufactured by HiMedia
Sourced in India
Petroleum ether is a solvent used in laboratory applications. It is a colorless, volatile, and flammable liquid mixture of aliphatic hydrocarbons. Petroleum ether is commonly used for extraction and purification processes in chemical and biological experiments.
Automatically generated - may contain errors
Lab products found in correlation
Hydrogen peroxide, by HiMedia (2 mentions)
Riboflavin, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Ethanol, by HiMedia (1 mentions)
Methanol, by HiMedia (1 mentions)
Sulphuric acid, by HiMedia (1 mentions)
Crystal violet, by HiMedia (1 mentions)
Safranin, by HiMedia (1 mentions)
Gelatin, by HiMedia (1 mentions)
Dntps, by Merck Group (1 mentions)
Kovacs reagent, by HiMedia (1 mentions)
Oxidase disc, by HiMedia (1 mentions)
Vp reagent, by HiMedia (1 mentions)
Bile salt, by Merck Group (1 mentions)
Bradford s reagent, by HiMedia (1 mentions)
Gram s iodine, by HiMedia (1 mentions)
Sodium thiosulphate, by HiMedia (1 mentions)
N butanol, by HiMedia (1 mentions)
Taq polymerase, by Merck Group (1 mentions)
3 protocols using petroleum ether
1
Antioxidant Activity Quantification
2
Extraction and Isolation of S. singueana Leaves
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Sufficient amounts of S. singueana leaves were collected in March 2013 from the northwest and central zones of Tigray, Northern Ethiopia. The plant material was authenticated at the National Herbarium, Department of Biology, Addis Ababa University (Addis Ababa, Ethiopia). Photographs of S. singueana taken during collection at the collection site and collected leaves are shown in Fig. 1 . The collected leaves were sorted, dried, powdered, and then defatted using petroleum ether (HiMedia Laboratories, Mumbai, India) for 48 hours. The marc was then allowed to dry and extracted three times by maceration using 70% ethanol with intermittent agitation. Each maceration was carried out for 72 hours. The extracts were filtered, collected, concentrated under reduced pressure using Rotavapor (BIBBY Sterlin Ltd, stuart®, UK), and dried in a vacuum oven at 35°C. The dried extracts were then transferred into vials and stored for further use.
3
Microbiology Lab Techniques Protocol
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Nutrient Agar, nutrient broth, luria bertani broth, muller hinton agar, glucose, dextrose, sucrose, lactose, mannitol, phenol red, starch agar, Gram's iodine, yeast extract, gelatin, skim milk powder, agar, peptone, tryptone, kovac's reagent, mr-vp media, simmons citrate agar, Nutrient Agar, nutrient broth, safranin, crystal violet, vp reagent (i), vp reagent (ii), oxidase disc, ferrous ammonium sulphate, sodium thiosulphate, potassium dihydrogen phosphate, Bradford's reagent, DNS reagent, hydrogen peroxide, petroleum ether, glacial acetic acid, n-Butanol were procured from Himedia Laboratories Pvt. Ltd. India. Trypsin, bile salts, Taq polymerase, dNTPs, α-Amylase procured from Sigma Aldrich, USA.
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