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Rtfpi

Manufactured by R&D Systems
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Sourced in United States

The RTFPI is a laboratory instrument designed for the rapid detection and quantification of transcription factor protein interactions. It utilizes a real-time fluorescence polarization-based assay to measure the binding of transcription factors to their target DNA sequences. The RTFPI provides a sensitive and reliable method for studying protein-DNA interactions in a high-throughput format.

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Lab products found in correlation

Rhace2, by R&D Systems (1 mentions) β actin antibody, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anti il 1β antibody, by Abcam (1 mentions) Anti gsdmd antibody, by Abcam (1 mentions) Anti pro caspase1 p10 p12 antibody, by Abcam (1 mentions) Mcc950, by MedChemExpress (1 mentions)

Close spelling variants of this product

Mouse rTFPI

2 protocols using rtfpi

1

SARS-CoV-2 Spike Protein Binding Analysis

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Recombinant SARS-CoV-2 S-proteins, SW, SD, rhACE2, rTFPI, and TM (BDCA3), were purchased from R&D Systems (Minneapolis, MN, USA). DTNB was from Sigma-Aldrich (St. Louis, MO, USA). Anti-human CD66b and anti-human CD45 antibodies were from Stemcell Technologies (Cambridge, MA, USA); anti-human CD16 antibodies were from Ancell Corporation (Stillwater, MN, USA). Monoclonal P-selectin antibodies and DAPI were from Thermo Fisher/Invitrogen (Waltham, MA, USA), and β-actin antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA).
Bhargavan B, & Kanmogne G.D. (2023). SARS-CoV-2 Spike Proteins and Cell–Cell Communication Induce P-Selectin and Markers of Endothelial Injury, NETosis, and Inflammation in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for the Pathogenesis of COVID-19 Coagulopathy. International Journal of Molecular Sciences, 24(16), 12585.
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2

GSDMD, Caspase-1, and IL-1β Detection

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Anti-GSDMD antibody, anti-pro-caspase-1 + p10 + p12 antibody, and anti-IL-1β antibody for western blotting were all purchased from Abcam (Cambridge, UK). Anti-NLRP3 antibody was purchased from Wanleibio (Shenyang, China). Anti-GSDMD antibody for immunofluorescence was purchased from Bioss (Beijing, China). MCC950 (NLRP3 inhibitor) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). rTFPI was purchased from R&D (Minneapolis, MN, USA).
Dai Y., Xu R., Yang K., Jiang T., Wei Y., Liu Y., Chen W., Fu Y, & Zhao Y. (2024). Effect of tissue factor pathway inhibitor on the pyroptosis of vascular smooth muscle cells induced by angiotensin II. Cardiovascular Diagnosis and Therapy, 14(1), 72-83.
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