Tri n butylamine

Tebuconazole (TEB), Ovalbumin (OVA), bovine serum albumin (BSA), N-(3-(dimethylamino)propyl)-N′-ethylcarbo-diimide hydrochloride (EDC·HCl), dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), tri-n-butylamine, isobutyl-chlorocarbonate, complete and incomplete Freund’s adjuvant, poly-ethylene glycol (PEG) 2000, hypoxanthine-aminopterin-thymidine (HAT) and hypoxanthine-thymidine (HT) medium, peroxidase-labeled goat antimouse IgGs, 3,3′,5,5′-tetramethylbenzidine (TMB), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA). Cell culture medium (DMEM) and fetal bovine serum were provided by Thermo Fisher Scientific (Waltham, MA, USA). TEB analogues (penconazole, propiconazole, myclobutanil, paclobutrazol, and hexaconazole) used for cross-reactivity studies were supplied by Aladdin Chemistry Co., Ltd. (Shanghai, China).
Quantum dot beads (QBs) were generated in Prof. Yonghua Xiong’s lab (Nanchang University, Nanchang, China).^{22 (link)} The elements of the immunochromatographic test strip platform comprising sample, absorbent pads, and NC membranes were purchased from Millipore Corp. (Bedford, MA, USA).

All hapten coupling reagents (sulfo-N-hydroxysuccinimide, N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, dicyclohexylcarbodiimide, isobutyl chloroformate, tri-n-butylamine), bovine serum albumin (BSA), thyroglobulin (Tg), goat anti-rabbit IgG peroxidase conjugate (GAR-HRP), Tween 20, and 3,3’,5,5’-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). ELISA was performed on 96-well microtiter plates (Nunc MaxiSorp, Roskilde, Denmark) and read spectrophotometrically with a microplate reader (Molecular Devices, Sunnyvale, CA) in dual wavelength mode (450 and 650 nm).