Acc1 ps79

Manufactured by Cell Signaling Technology

ACC1-pS79 is a laboratory product offered by Cell Signaling Technology. It is an antibody that specifically recognizes the phosphorylation of Serine 79 on the Acetyl-CoA Carboxylase 1 (ACC1) protein.

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2 protocols using acc1 ps79

Comprehensive Lipid Metabolism Signaling Pathway

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Antibodies to ACC1-pS79, ACC1, FASN, SCD1, Tubulin, TBP, GAPDH, AKT-pS473, AKT, GSK3β-pS21/9, GSK3β, TSC2-pT1462, TSC2, mTOR-pS2448, mTOR, S6K-pT389, S6K, S6-pS240/244, S6, 4E-BP1-pS65, 4E-BP1, β-actin, LRP6-pS1490, LRP6, TCF7L2, β-catenin, β-catenin-pS33/37/T41, IGF1R, AKT-pT308, and Lamin B were purchased from Cell Signaling. Antibodies to apoB, SCAP, Insig1, Insig2, Sp5, Sp1 were purchased from Santa Cruz Biotech (Santa Cruz, Ca, USA). Antibodies to MTP, SREBP1, SREBP2, IRS1 were purchased from BD Biosciences. Antibodies to ACAT2 and IGF1 were purchased from Novus. Antibodies to ELOVL6 (Thermo Scientific), DGAT1 (Bio Vision), GPAT1 (GeneTex), LXRα (Abcam), HMGCR (Upstate), and IRS1-pY612 (Invitrogen) were used for western blotting.

Go G.W., Srivastava R., Hernandez-Ono A., Gang G., Smith S.B., Booth C.J., Ginsberg H.N, & Mani A. (2014). The Combined Hyperlipidemia Caused by Impaired Wnt-LRP6 signaling is reversed by Wnt3a Rescue. Cell metabolism, 19(2), 209-220.

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Immunoblotting Protein Detection Workflow

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Immunoblotting was performed as described previously (6, 24 (link)). Blots were probed with Streptavidin-HRP (Cell Signaling Technology #3999; RRID:AB_10830897) or antibodies used according to manufacturer's recommendations: WNT4 (R&D Systems, MAB4751; RRID:AB_2215448); WNT3A (Novus Biologicals, MAB13242); anti-HA-HRP conjugate (Cell Signaling Technology #2999; RRID:AB_1264166); DHRS2 (Sigma HPA053915; RRID:AB_2682307); mTOR (Cell Signaling Technology #2983; RRID:AB_2105622); STAT1 (Sigma HPA000931; RRID:AB_1080100).; MCL1 (Cell Signaling Technology #5453; RRID:AB_10694494); ACC1 (ACACA, Abcam ab45174; RRID:AB_867475); ACC1-pS79 (Cell Signaling Technology #3661; RRID:AB_330337); AMPKα (Cell Signaling Technology #2532; RRID:AB_330331); AMPKα-pT172 (Cell Signaling Technology #2535; RRID:AB_331250). We note that recent lots of WNT4 MAB4751 show substantially increased non-specific background relative to our prior studies (6, 24 (link)), and can detect overexpressed WNT4 but no longer reliably detect endogenous WNT4 protein.

Sottnik J.L., Shackleford M.T., Robinson S.K., Villagomez F.R., Bahnassy S., Oesterreich S., Hu J., Madak-Erdogan Z., Riggins R.B., Corr B.R., Cook L.S., Treviño L.S., Bitler B.G, & Sikora M.J. (2024). WNT4 Regulates Cellular Metabolism via Intracellular Activity at the Mitochondria in Breast and Gynecologic Cancers. Cancer Research Communications, 4(1), 134-151.

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