MDA-MB-231 and MCF-7 cell lines were acquired from the American Type Culture Collection (ATCC) and MCF-10A was purchased from Iranian Biological Resource Center (IBRC) (Tehran, Iran). MDA-MB-231was cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 (1:1) enriched with 100 U/ml penicillin and 100 μg/ml Streptomycin, 10% fetal bovine serum (FBS; Gibco), and L-glutamine (6 mM). MCF7 was cultivated in minimal essential medium (MEM) containing 100 U/ml penicillin and 100 μg/ml Streptomycin, 10% fetal bovine serum (FBS; Gibco). MCF-10A was maintained in DMEM/F12 (1:1) containing 5% horse serum, 20 ng/mL EGF, 10 μg/mL insulin, 50 μg/mL hydrocortisone and 100 U/ml penicillin and 100 μg/ml Streptomycin. All cells were incubated at 37 °C in a humidified atmosphere containing 5% CO2.
Mcf 10a
Manufactured by Iranian Biological Resource Center
The MCF-10A is a cell line derived from human breast epithelial cells. It is a non-tumorigenic, immortalized cell line commonly used in cell biology research. The MCF-10A cell line retains many characteristics of normal breast epithelial cells and is a widely-used model system for studying cellular processes such as proliferation, differentiation, and response to various stimuli.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231, by American Type Culture Collection (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by Iranian Biological Resource Center (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Iranian Biological Resource Center (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Ecori, by Thermo Fisher Scientific (1 mentions)
Kmno4, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Xmark microplate spectrophotometer, by Bio-Rad (1 mentions)
Gcms qp2010, by Shimadzu (1 mentions)
3 protocols using mcf 10a
1
Culturing Breast Cancer Cell Lines
2
miR-101 Delivery and Evaluation in Breast Cancer
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Hsa-miR-101 and the scramble (5-miR-101) labeled by cyanine 5 (cy5) fluorescent dye were prepared from TAG Copenhagen (Denmark). High-purity graphite powder (,45 μm), poly-l -arginine (70 KD), polyethyleneglycol bis amin (3 KD), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), fluorescein isothiocyanate (FITC), and N-hydroxysuccinimide (NHS) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Sulfuric acid (H2SO4; 98%), NaNo3, KMnO4, HCL, and DMSO were purchased from Merck & Co., Inc. (Whitehouse Station, NJ, USA). 1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) was obtained from Acros (NJ, NY, USA). EcoRI and BamH1 restriction enzymes were prepared from Thermo Fisher Scientific (Waltham, MA, USA). Beta-actin antibody (mouse monoclonal), stathmin1 antibody (rabbit polyclonal), and HRP polyclonal secondary antibody were purchased from Abcam (Cambridge, UK). MDA-MB-231 (basal aggressive metastatic, estrogen receptor–negative breast epithelial adenocarcinoma), MCF7 (luminal non-metastatic, estrogen and progesterone receptor–positive breast epithelial adenocarcinoma), MCF10A (immortal and non-transformed epithelial human breast), and HU-02 (primary human foreskin fibroblast) were obtained from the Iranian Biological Resource Center (Tehran, Iran).
3
GC-MS Analysis and α-Glucosidase Inhibition
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The MDA-MB-231 and MCF-7 cell lines were acquired from the American Type Culture Collection (ATCC) and MCF-10A was purchased from the Iranian Biological Resource Center (IBRC) (Tehran, Iran). GC-MS was conducted on a gas chromatography-mass spectrometer (GC-MS-QP2010, Shimadzu Kyoto, Japan). The α-glucosidase enzyme (EC 3.2.1.20, Sigma-Aldrich, Darmstadt, Germany) and spectrophotometer (xMark™ Microplate Spectrophotometer, Bio-Rad, Hercules, CA, USA) were used for the α-glucosidase activity. A High-Speed Multifunctional Grander (Grand Household, Code. GR-SCG350H) was used for the grinding of the plant. Analytical grade reagents were used in the current study.
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