BCDX2, fluorescently labelled BAF647CDX2 or BCDX2AF647, was mixed with either dN15nt, 5′-Cy3-dN15nt or dN15nt-Cy3-3′ ssDNA in 10 μL of 25 mM HEPES pH 7.5, 5% glycerol, 2.5 mM MgCl2, 100 mM NaCl, 0.25 mM TCEP, 1 mM ADP.AlFx (1 mM ADP,1 mM AlCl3, 10 mM NaF) in 384 well low volume microplates (Corning). Fluorescence emission spectra from 550 nm to 800 nm were measured following excitation at 500 nm on a Clariostar plate reader (BMG Labtech). The ratio of fluorescence intensity (IA/ID) at 647 nm and 555 nm was measured for all combinations of protein and ssDNA.
Low volume microplates
Manufactured by Corning
Low volume microplates are a type of laboratory equipment designed for handling small sample volumes. They provide a convenient platform for various experiments and assays that require miniaturized sample sizes. The core function of low volume microplates is to facilitate the efficient and precise handling of minute liquid volumes, typically ranging from a few microliters to a few hundred microliters, in a standardized multi-well format.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using low volume microplates
1
Fluorescence-based Protein-DNA Binding Assay
2
Fluorescence-based Protein-DNA Binding Assay
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