Anti β tubulin hc101

Met was purchased from Solarbio (Beijing, China). Staurosporine (STS), Caspase-3/8/9 Assay Kit, ROS Assay Kit and JC-1 Assay Kit were provided by Beyotime (Shanghai, China). Anti-PARP (9532), anti-Bak (12105), anti-Bax (5023), anti-Bcl-xL (2764), anti-Mcl-1 (94296), anti-Bim (2933), anti-Bad (9268), anti-Puma (98672), anti-AMPK (5831), anti-p-AMPK (8208), anti-Cleaved Caspase-3 (9664), anti-Cleaved Caspase-8 (8592), anti-Caspase-9 (9502) Rabbit monoclonal antibody, and anti-Bid (8762) Mouse monoclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH (sc-47724) Mouse monoclonal antibody was obtained from Santa Cruz (Texas, USA). Luciferase Mycoplasma Detection Kit and anti-β-tubulin (HC101) Mouse monoclonal antibody were purchased from Transgen Biotech (Beijing, China). Turbofect™ transfection regent and Mito-tracker Deep Red dye were obtained from ThermoFisher Scientific (Massachusetts, USA). Cell counting Kit-8 (CCK-8) and Alexa Fluor^® 488 annexin V/Dead Cell Apoptosis Kit were purchased from Dojindo (Kyushu, Japan). Acetylcysteien (NAC), Z-VAD-(OMe)-FMK (Z-VAD) and Compound C were purchased from MedChemExpress (New Jersey, USA). Hoechst 33258 dye and PI dye were obtained from G-clone (Beijing, China).

Recombinant Human Wnt-3a (NP-149122) was purchased from an R&D system (Minneapolis, MN, USA).
Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-β-actin (A1978), anti-rabbit-IgG-HRP (AP132P), and anti-mouse-IgG-HRP (AP308P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). anti-c-Myc (HT101) and anti-β-tubulin (HC101) were purchased from TransGen Biotech (Beijing, China). anti-β-Catenin (8480) and anti-non-phospho β-Catenin (19807) were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). anti-LEF1 (sc-374522) and anti-Arc (sc-17839) were purchased from Santa Cruz Biotechnology (San Diego, CA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from A549 cDNA and subcloned into the pcDNA3.1 vector. The plasmids were confirmed by DNA sequencing at Sangon Biotech (Shanghai, China).