All cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, MD). WHIM 3 is a breast cancer cell line that was derived from a patient-derived xenograft.24 (link) WHIM 3 was provided by the HAMLET core at WUSM. All cell lines were maintained in RPMI or DMEM Supplemented with 10% FBS and antibiotics (GIBCO BRL, Grand Island, NY). MCF-10A cells were grown in MEGM Mammary Epithelial Cell Growth Medium (Lonza, Allendale, NJ) Supplemented with 5% Horse serum. Recombinant EGF, TGFβ and TNFα were purchased from R&D Systems (Minneapolis, MN). The MAP kinase inhibitors U0126, PD98059, SB203580, SP600125, and LY29004 were purchased from Selleck Chemicals (Houston, TX).
Ly29004
Manufactured by Selleck Chemicals
Sourced in United States
LY29004 is a chemical compound used in laboratory research. It functions as a phosphoinositide 3-kinase (PI3K) inhibitor. The core function of LY29004 is to inhibit the activity of PI3K, an enzyme involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Sp600125, by Selleck Chemicals (2 mentions)
Sb203580, by Selleck Chemicals (2 mentions)
Recombinant egf, by R&D Systems (1 mentions)
Megm mammary epithelial cell growth medium, by Lonza (1 mentions)
U0126, by Selleck Chemicals (1 mentions)
Pd98059, by Selleck Chemicals (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Ci 1040, by Selleck Chemicals (1 mentions)
Recombinant epidermal growth factor egf, by R&D Systems (1 mentions)
Transforming growth factor β tgf β, by R&D Systems (1 mentions)
Mcf 10a, by Lonza (1 mentions)
Mcf10a cell lines, by American Type Culture Collection (1 mentions)
Mammary epithelial cell growth medium, by Lonza (1 mentions)
2 protocols using ly29004
1
Patient-derived Breast Cancer Cell Line Characterization
2
MCF-10A Cell Culture and Inhibitors
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MCF-10A cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). MCF-10A cells were grown in Mammary Epithelial Cell Growth Medium (Lonza, Allendale, NJ, USA) supplemented with 5% horse serum. Recombinant epidermal growth factor (EGF) and transforming growth factor-β (TGFβ) were purchased from R&D Systems (Minneapolis, MN, USA).
The MAPK inhibitors CI-1040, SB203580, and SP600125 and AKT inhibitor LY29004 were purchased from Selleck Chemicals (Houston, TX, USA). BAY117082, dasatinib, doxorubicin, etoposide, paclitaxol and quercitin were purchased from LC labs (Woburn, MA, USA).
The MAPK inhibitors CI-1040, SB203580, and SP600125 and AKT inhibitor LY29004 were purchased from Selleck Chemicals (Houston, TX, USA). BAY117082, dasatinib, doxorubicin, etoposide, paclitaxol and quercitin were purchased from LC labs (Woburn, MA, USA).
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