Phospho t73 rab10

Manufactured by Abcam

Sourced in United Kingdom

Phospho-T73-Rab10 is a phospho-specific antibody that recognizes Rab10 protein when phosphorylated at threonine 73. This antibody can be used to detect and quantify the phosphorylation state of Rab10 in biological samples.

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Lab products found in correlation

Anti egfp antibody ab6673, by Abcam (2 mentions) Anti myc antibody, by Abcam (2 mentions) Anti flag m2, by Merck Group (2 mentions) Total lrrk2 mjff2 c41 2, by Abcam (1 mentions) Tyrosine hydroxylase, by Merck Group (1 mentions) Darpp32, by Merck Group (1 mentions) β tubulin 3, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) Ab22048, by Abcam (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) β actin sc 47778hrp, by Santa Cruz Biotechnology (1 mentions) Chemidoc touch, by Bio-Rad (1 mentions)

Close spelling variants of this product

Rab10

3 protocols using phospho t73 rab10

Antibody Detection of LRRK2 and Rab10

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For western blotting the following antibodies were used: total LRRK2 [MJFF2 (c41-2)] (Abcam, Cambridge, UK, Cat# ab133474, 1:300), phospho-S935 LRRK2 (Abcam, Cambridge, UK, Cat# ab133450, 1:500), phospho-S1292 LRRK2 (Abcam, Cambridge, UK, Cat# ab203181, 1:300), total Rab10 (Abcam, Cambridge, UK, Cat# ab104859, 1:500), phospho-T73 Rab10 (Abcam, Cambridge, UK, Cat# ab230261, 1:400), tyrosine hydroxylase (Millipore, Burlington, MA, USA Cat# AB152, 1:10000), DARPP32 (Millipore, Burlington, MA, USA Cat# AB10518, 1:10000) and β-actin (Sigma-Aldrich, St. Louis, MO, USA Cat# A2066, 1:10000). For immunofluorescence: β-tubulin-III (Sigma-Aldrich, St. Louis, MO, USA, Cat# T8578, 1:1000); total LRRK2 [MJFF2 (c41-2)] (Abcam, Cambridge, UK, Cat# ab133474, 1:200), phospho-S935 LRRK2 [UDD2 10(12)] (Abcam, Cambridge, UK, Cat# ab172382, 1:200), GLT1 (EMD Millipore, Burlington, MA, USA Cat# AB1783, 1:400), GFAP (Dako-Agilent, Santa Clara, CA, USA, Cat# Z0334, 1:400) CD11b [M1/70] (eBioscience™ from Thermo Fisher Scientific, Waltham, MA, USA, Cat# 14-0112-82, 1:200).

Iannotta L., Biosa A., Kluss J.H., Tombesi G., Kaganovich A., Cogo S., Plotegher N., Civiero L., Lobbestael E., Baekelandt V., Cookson M.R, & Greggio E. (2020). Divergent Effects of G2019S and R1441C LRRK2 Mutations on LRRK2 and Rab10 Phosphorylations in Mouse Tissues. Cells, 9(11), 2344.

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Immunofluorescence Staining of LRRK2 and Rab10

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Cells are fixed with 4% paraformaldehyde, washed 3 time with phosphate-buffered saline(PBS), permeabilized with PBS supplemented with 0.1% saponin, blocked with 5% goat serum and immunestained for 12 hours with primary antibodies followed by 12hours staining with secondary antibody. Horchest is used for nucleus staining. For confocal microscopy or airyscan microscopy, cell culture coverslips are mounted on glass slides with ProLong Gold antifade mountant (Thermo Fisher Scientific).
The following antibodies were used: N241A/34 anti-LRRK2 (Antibodies Inc), phospho-T73-Rab10(MJF-R21-22-5), total RAB10 antibody (MJF-R23), anti-eGFP antibody (ab6673, Abcam), anti-FLAG M2 (Sigma), anti-Myc antibody (ab32 Abcam), EEA1(sc-365652, santa cruz), LAMP1(sc-19992), CD11b(14-0112-82, Thermo Fisher Scientific), TLR4 (ab22048, Abcam), CCR5 (17-1951-80 Thermo Fisher Scientific).

Liu Z., Xu E., Zhao H., Cole T., & West A.B. (2020). LRRK2 and Rab10 Coordinate Macropinocytosis to Mediate Immunological Responses in Phagocytes.

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Immunoblotting Analysis of LRRK2 and Rab10 Signaling

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Protein lysates were analyzed using SDS-PAGE followed by transfer to PVDF membranes for immunoblotting with indicated primary antibodies and HRP-conjugated secondary antibodies. Signals developed with Classico ECL reagent (Millipore) was digitally detected using Chemidoc Touch (BioRad).
Intensities of the indicated bands are calculated with ImageLab software. The following antibodies were used: N241A/34 anti-LRRK2 (Antibodies Inc), phospho-T73-Rab10(MJF-R21), anti-eGFP antibody (ab6673, Abcam), total Rab10 antibody (Cell signaling), anti-FLAG M2 (Sigma), anti-Myc antibody (ab32 Abcam), p38 antibody (8690, cell signaling), phospho-p38 antibody (4511, cell signaling), IRF3 antibody(4302, cell signaling), phospho-IRF3 antibody(4947 cell signaling), total AKT antibody(4691), phospho-AKT antibody(4060), β-actin (sc-47778 HRP, Santa Cruz).

Liu Z., Xu E., Zhao H., Cole T., & West A.B. (2020). LRRK2 and Rab10 Coordinate Macropinocytosis to Mediate Immunological Responses in Phagocytes.

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