Cgk733

Manufactured by Abcam

Sourced in United Kingdom

CGK733 is an antibody designed to detect the human Sodium/potassium-transporting ATPase subunit alpha-1 (ATP1A1) protein. It is intended for use in various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Infinite m200, by Tecan (1 mentions) Nu7026, by Abcam (1 mentions) Strophanthidin, by Merck Group (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Abcam (1 mentions)

2 protocols using cgk733

Cell Viability Assay for NU7026 and CGK733

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MRC-5 and A549 cells were plated into 96-well dishes at a density of 5×10⁴ cells/well. NU7026 and CGK733 (Abcam, Cambridge, UK) were added to each well at a final concentration of 5–50 µM, and incubated for 48 h. Thereafter, MTT (final concentration, 5 mg/ml) was added to each well. The medium was then removed, and the formazan crystals were dissolved by adding 150 µl dimethyl sulfoxide. The absorbance at 490 nm was subsequently measured in a microplate reader (Infinite M200; Tecan Group Ltd., Männedorf, Switzerland) (16 (link),17 (link)).

YANG L., LIU Y., SUN C., YANG X., YANG Z., RAN J., ZHANG Q., ZHANG H., WANG X, & WANG X. (2015). Inhibition of DNA-PKcs enhances radiosensitivity and increases the levels of ATM and ATR in NSCLC cells exposed to carbon ion irradiation. Oncology Letters, 10(5), 2856-2864.

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Compound Preparation for Cell Assays

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FCCP and CGK-733 were purchased from Abcam (Cambridge, MA), and pyrvinium pamoate was purchased from USP (Rockville, MD). These three compounds were dissolved in dimethyl sulfoxide (DMSO) at stock solutions of 10 mM. Strophanthidin was purchased from Sigma-Aldrich (St Louis, MO) and was dissolved in 100% ethanol at a stock solution of 10 mM. All stock solutions were stored at −20 °C for subsequent use in cell-based experiments.

Chen B., Ma L., Paik H., Sirota M., Wei W., Chua M.S., So S, & Butte A.J. (2017). Reversal of cancer gene expression correlates with drug efficacy and reveals therapeutic targets. Nature Communications, 8, 16022.

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