The LGTV Envelope domain EDIII gene was amplified from nymphs’ cDNA and cloned into the pET-28a(+) expression vector [24 (link)]. A rabbit polyclonal anti-EDIII antibody was generated against recombinant EDIII protein (rec EDIII) in GL Biochem, Shanghai, China [25 (link)]. To determine the specificity of the anti-LGTV antibody, E protein levels were compared between ticks and cells infected with or without LGTV. Mouse anti-β-Actin antibody (1:2000) (Abbkine, Wuhan, China) was used as an internal control.
Mouse anti β actin antibody
Manufactured by Abbkine
Sourced in China
The Mouse anti-β-Actin antibody is a primary antibody that recognizes the β-actin protein, a key structural component of eukaryotic cells. This antibody can be used for the detection and quantification of β-actin in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.
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Lab products found in correlation
2 protocols using mouse anti β actin antibody
1
Cloning and Antibody Production for LGTV EDIII
2
Immunoblotting of Midgut Proteins
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Proteins of 10 midguts 24 h post blood meal were extracted in 300 µl SDS/urea lysis buffer (8 M urea, 2 % SDS, 5 % β-mercaptoethanol, 125 mM Tris-HCl).
Immunoblotting was performed using standard procedures using the antibodies rabbit anti-Per1 antibody (1:5000) and mouse anti-β-Actin antibody (1:2000)
(Abbkine, China). The rabbit polyclonal anti-Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55-462 of per1 CDS (Aste010406) expressed in pET-42a (Novagen) commercially (GL Biochem Ltd, Shanghai, China).
Immunoblotting was performed using standard procedures using the antibodies rabbit anti-Per1 antibody (1:5000) and mouse anti-β-Actin antibody (1:2000)
(Abbkine, China). The rabbit polyclonal anti-Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55-462 of per1 CDS (Aste010406) expressed in pET-42a (Novagen) commercially (GL Biochem Ltd, Shanghai, China).
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