Antibodies against flag f1804

Manufactured by Merck Group

Anti-FLAG antibodies (F1804) are a laboratory reagent used to detect and purify proteins that have been engineered to contain a short FLAG peptide sequence. These antibodies specifically bind to the FLAG tag, allowing for the identification and isolation of the tagged proteins from complex mixtures.

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Lab products found in correlation

Ez link hpdp biotin, by Thermo Fisher Scientific (1 mentions) Dmso d2650, by Merck Group (1 mentions) Tmz hy 17364, by MedChemExpress (1 mentions) Rhodamine phalloidin phdr1, by Cytoskeleton (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Sumo1, by Cell Signaling Technology (1 mentions) Anti senp1 ab108981, by Abcam (1 mentions) Sumo2 3, by Cell Signaling Technology (1 mentions) Parp1, by Cell Signaling Technology (1 mentions) Cleaved caspase 8, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

F1804 (465 citations) FLAG (F1804) (21 citations) Antibodies against Flag (9 citations)

2 protocols using antibodies against flag f1804

Protein Profiling of Cellular Signaling

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Antibodies against GSK3β (12456), p-GSK3β (S9) (8566), GSK3α (4337), p-GSK3α (S21) (9316), EZH2 (5246), STAT3 (9139), p-STAT3 (Y705) (9145), β-catenin (8480), AKT1 (4691), and HA (3724) were purchased from Cell Signaling Technology. Antibodies against GFP (50430), β-tubulin (66031), PKA (55382), p70S6K (14485), p90RSK (16463), and MGMT (17195) were purchased from Proteintech (Wuhan, China). ZDHHC4 (ab235369), p-GSK3α + β (Y216 + Y279) (ab68476), and p-EZH2 (S21) (ab84989) were obtained from Abcam. Antibodies against FLAG (F1804) were purchased from Sigma–Aldrich. Methyl K. AlexaFluor488 goat anti-rabbit IgG (A11008) and AlexaFluor568 goat anti-rabbit IgG (A11011) were purchased from Invitrogen (Carlsbad, CA, USA). Rhodamine phalloidin (#PHDR1) was purchased from Cytoskeleton (USA). Protein chips were obtained from R&D Systems (USA).
TMZ (HY-17364) and Niclosamide (HY-B0497) were purchased from MedChemExpress (Shanghai, China). N-ethylmaleimide (NEM) (23030), hydroxylamine HCl (HAM) (26103), and EZ-Link™ HPDP-Biotin (A35390) were obtained from Thermo Fisher. DMSO (D2650) was purchased from Sigma–Aldrich.

Zhao C., Yu H., Fan X., Niu W., Fan J., Sun S., Gong M., Zhao B., Fang Z, & Chen X. (2022). GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2–STAT3 axis. Oncogenesis, 11(1), 28.

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Vinculin Antibody Immunoprecipitation Protocol

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Antibodies against vinculin (sc-7649) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Flag M2 beads (M8823) and antibodies against Flag (F1804) and HA (H6908) were from Sigma-Aldrich. Antibody against RGS-His (34610) was purchased from Qiagen. SUMO1 (#4930), SUMO2/3 (#4971), PARP-1 (#9532), cleaved-caspase 3 (#9661), and cleaved-caspase 8 (#18C8) were purchased from Cell Signaling Technology. Anti-SENP1 (ab108981) was from Abcam. Lipofectamine 2000 (11668-019) was purchased from Invitrogen. Momordin Ιc (purity > 98%, CAS:96990-18-0) was purchased from Chengdu Finn Biotechnology Co., LTD.

Wu J., Lei H., Zhang J., Chen X., Tang C., Wang W., Xu H., Xiao W., Gu W, & Wu Y. (2016). Momordin Ic, a new natural SENP1 inhibitor, inhibits prostate cancer cell proliferation. Oncotarget, 7(37), 58995-59005.

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