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Pe cy7 cd25 ab

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PE-Cy7-CD25 Ab is a fluorescent-labeled antibody that binds to the CD25 protein. CD25 is a cell surface marker expressed on activated T cells and regulatory T cells. This product can be used in flow cytometry applications for the detection and analysis of CD25-positive cells.

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Lab products found in correlation

Apc cy7 cd8 ab, by BD (2 mentions) Percp cy5.5 cd62l ab, by Thermo Fisher Scientific (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Lsr 2, by BD (2 mentions)

2 protocols using pe cy7 cd25 ab

1

Multiparametric Analysis of Allograft Rejection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen, kidney, and kidney draining lymph nodes were harvested from rejecting and long-term mice (> 200 days). Single cells were prepared from the spleen and lymph nodes by gentle mechanical dispersion and from islet-harboring kidneys by collagenase digestion. Cells were stained using antibodies to cell surface markers (Alexa 700-CD4 Ab 1:200 BD Bioscience 557956, APC-Cy7-CD8 Ab 1:100 BD Bioscience 557654, PE-Cy7-CD25 Ab 1:100 from Pharmingen 25-0251-82, BD, and eFlour 450-CD44 Ab 1:100 eBiosicences 48-0441-82 and PerCP-Cy5.5- CD62L Ab at 1:200 from eBioscience 45-0621-82). Intracellular FoxP3 staining was carried out on fixed/permeablized cells using FoxP3 Transcription Factor Staining Buffer set (diluted 1:20, eBioscience 11-5773-82). Data was collected using BD LSR II and analyzed using Diva software and FlowJo 9.8. Data was graphed using GraphPad Prism and t tests with Welch’s correction were used to determine significance between groups, p <0.05 was considered significant.
Headen D.M., Woodward K.B., Coronel M.M., Shrestha P., Weaver J.D., Zhao H., Tan M., Hunckler M.D., Bowen W.S., Johnson C.T., Shea L., Yolcu E.S., García A.J, & Shirwan H. (2018). Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nature materials, 17(8), 732-739.
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2

Multiparametric Analysis of Allograft Rejection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen, kidney, and kidney draining lymph nodes were harvested from rejecting and long-term mice (> 200 days). Single cells were prepared from the spleen and lymph nodes by gentle mechanical dispersion and from islet-harboring kidneys by collagenase digestion. Cells were stained using antibodies to cell surface markers (Alexa 700-CD4 Ab 1:200 BD Bioscience 557956, APC-Cy7-CD8 Ab 1:100 BD Bioscience 557654, PE-Cy7-CD25 Ab 1:100 from Pharmingen 25-0251-82, BD, and eFlour 450-CD44 Ab 1:100 eBiosicences 48-0441-82 and PerCP-Cy5.5- CD62L Ab at 1:200 from eBioscience 45-0621-82). Intracellular FoxP3 staining was carried out on fixed/permeablized cells using FoxP3 Transcription Factor Staining Buffer set (diluted 1:20, eBioscience 11-5773-82). Data was collected using BD LSR II and analyzed using Diva software and FlowJo 9.8. Data was graphed using GraphPad Prism and t tests with Welch’s correction were used to determine significance between groups, p <0.05 was considered significant.
Headen D.M., Woodward K.B., Coronel M.M., Shrestha P., Weaver J.D., Zhao H., Tan M., Hunckler M.D., Bowen W.S., Johnson C.T., Shea L., Yolcu E.S., García A.J, & Shirwan H. (2018). Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nature materials, 17(8), 732-739.
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