Sirna id s115

Manufactured by Thermo Fisher Scientific

SiRNA ID s115 is a synthetic, double-stranded RNA molecule designed for gene silencing applications. It is intended to be used as a research tool to modulate the expression of specific genes in cell-based studies.

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Lab products found in correlation

Nt sirna, by Thermo Fisher Scientific (2 mentions) 4d nucleofector system, by Lonza (1 mentions) P3 primary cell solution, by Lonza (1 mentions)

2 protocols using sirna id s115

Nucleofection of Human CD34+ Cells

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Human CD34+ cells were transfected by using the 4D-Nucleofector System (LONZA) as previously reported^{43 (link)}. Briefly, starting from the day after CD34+ cell purification, each sample was electroporated three times, once every 24 hours, with a small interfering RNA (siRNA) targeting human OXR1 mRNA (LIFE TECHNOLOGIES, siRNA ID s115). To exclude non-specific effects caused by interfering RNA (RNAi) nucleofection, a sample transfected with a nontargeting siRNA (NTsiRNA; LIFE TECHNOLOGIES) was included. Cells were analyzed 48 h after the last nucleofection for both cell viability and OXR1 mRNA expression.

Salati S., Genovese E., Carretta C., Zini R., Bartalucci N., Prudente Z., Pennucci V., Ruberti S., Rossi C., Rontauroli S., Enzo E., Calabresi L., Balliu M., Mannarelli C., Bianchi E., Guglielmelli P., Tagliafico E., Vannucchi A.M, & Manfredini R. (2019). Calreticulin Ins5 and Del52 mutations impair unfolded protein and oxidative stress responses in K562 cells expressing CALR mutants. Scientific Reports, 9, 10558.

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Silencing CALR in Human CD34+ Cells

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Human CD34 + cells were transfected by using the 4D-NucleofectorÔ System (Lonza) as previously reported [15] . Briefly, starting from the day after CD34 + cell purification, each sample was electroporated thrice, once every 24 h, with a small interfering RNA (siRNA) targeting human CALR mRNAs (Life Technologies, siRNA ID s115). For each electroporation, 4 • 10 5 CD34 + cells were resuspended in 100 mL of P3 Primary Cell Solution (Lonza), containing 3 mg of siRNA, and pulsed with the program DS112. To exclude nonspecific effects caused by interfering RNA (RNAi) nucleofection, a sample transfected with a nontargeting siRNA (NTsiRNA; Life Technologies) was included. Cells were analyzed 48 h after the last nucleofection for both cell viability and CALR mRNA and protein expression.

Salati S., Prudente Z., Genovese E., Pennucci V., Rontauroli S., Bartalucci N., Mannarelli C., Ruberti S., Zini R., Rossi C., Bianchi E., Guglielmelli P., Tagliafico E., Vannucchi A.M, & Manfredini R. (2018). Calreticulin Affects Hematopoietic Stem/Progenitor Cell Fate by Impacting Erythroid and Megakaryocytic Differentiation. Stem cells and development, 27(4).

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