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Iox 2

Manufactured by EMKA Technologies
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Sourced in France

The IOX 2.10 is a laboratory equipment product from EMKA Technologies. It is designed for core functions related to scientific research and analysis. No further details on its intended use are provided.

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Lab products found in correlation

Hrv software, by Kubios (1 mentions)

6 protocols using iox 2

1

Ventilatory Flow Dynamics Analysis

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Ventilatory flow signals were recorded, amplified, digitized and analyzed using iOX 2.10 (EMKA Technologies) to determine ventilatory parameters over sequential 20 s epochs (~50 breaths), during periods of behavioral quiescence and regular breathing. Minute ventilation (VE, ml/min/g) was calculated as the product of respiratory frequency (fR, breaths/min) and tidal volume (VT, ml/breath), and normalized to body weight (g). For analysis of the basal VE in hyperoxia, or acute hypercapnic ventilatory response, we sampled 10 consecutive epochs (200 s, representing ~400–500 breaths at rest) that showed the least inter-breath irregularity during the steady-state plateau period after each CO2 exposure, as determined by Poincaré analysis. For neonates, we obtained measurements from periods of time when the mice were visibly quiescent. The response to hypoxia was determined from the peak VE during the hypoxic exposure (from a 20 s epoch at ~30 s to 1 min) 12 (link). Analysis was performed by individuals blinded to treatment and/or genotype.
Shi Y., Stornetta D.S., Reklow R.J., Sahu A., Wabara Y., Nguyen A., Li K., Zhang Y., Perez-Reyes E., Ross R.A., Lowell B.B., Stornetta R.L., Funk G.D., Guyenet P.G, & Bayliss D.A. (2020). A brainstem peptide system activated at birth protects postnatal breathing. Nature, 589(7842), 426-430.
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2

Ventilatory Flow Dynamics Analysis

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Ventilatory flow signals were recorded, amplified, digitized and analyzed using iOX 2.10 (EMKA Technologies) to determine ventilatory parameters over sequential 20 s epochs (~50 breaths), during periods of behavioral quiescence and regular breathing. Minute ventilation (VE, ml/min/g) was calculated as the product of respiratory frequency (fR, breaths/min) and tidal volume (VT, ml/breath), and normalized to body weight (g). For analysis of the basal VE in hyperoxia, or acute hypercapnic ventilatory response, we sampled 10 consecutive epochs (200 s, representing ~400–500 breaths at rest) that showed the least inter-breath irregularity during the steady-state plateau period after each CO2 exposure, as determined by Poincaré analysis. For neonates, we obtained measurements from periods of time when the mice were visibly quiescent. The response to hypoxia was determined from the peak VE during the hypoxic exposure (from a 20 s epoch at ~30 s to 1 min) 12 (link). Analysis was performed by individuals blinded to treatment and/or genotype.
Shi Y., Stornetta D.S., Reklow R.J., Sahu A., Wabara Y., Nguyen A., Li K., Zhang Y., Perez-Reyes E., Ross R.A., Lowell B.B., Stornetta R.L., Funk G.D., Guyenet P.G, & Bayliss D.A. (2020). A brainstem peptide system activated at birth protects postnatal breathing. Nature, 589(7842), 426-430.
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3

Rat Pulmonary Function Assessment

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Lung function was assessed as described previously51 . Respiratory function was measured using a pulmonary function testing system (Emka Technologies, Paris, France), and the raw data were converted using specialized analysis software (IOX 2.9.4.32, Emka Technologies). Briefly, conscious rats were placed in plethysmography chambers for more than 15 minutes, eliminate the data animals adapt to their environments or sleep. The measured indices included inspiratory time, expiratory time, peak inspiratory flow, peak expiratory flow, tidal volume, expiratory volume, relaxation time, minute respiratory volume, end-inspiratory/expiratory pause and respiratory frequency.
Yan X.D., Wang Q.M., Tie C., Jin H.T., Han Y.X., Zhang J.L., Yu X.M., Hou Q., Zhang P.P., Wang A.P., Zhang P.C., Gao Z, & Jiang J.D. (2017). Polydatin protects the respiratory system from PM2.5 exposure. Scientific Reports, 7, 40030.
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4

Respiratory Function Assessment in Rats

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Respiratory function was measured using a whole body plethysmography system (Emka Technologies, Paris, France) and the raw data were converted using specialized analysis software (IOX 2.9.4.32, Emka Technologies). Briefly, conscious rats were placed in plethysmography chambers for 10–15 min, eliminating data from animals adapting to their environments or sleeping. The test parameters, including respiratory rate (f), expiratory flow at 50% tidal volume (EF50), peak expiratory flow (PEF), peak inspiratory flow (PIF), tidal volume (TV), minute ventilation volume (MV), expiratory volume (EV), inspiratory time (Ti), relaxation time (RT) and expiratory time (Te), were recorded during a 3–8 min period once a week for 11 weeks [56 (link)].
Liu T., Zhang P., Ling Y., Hu G., Gu J., Yang H., Wei J., Wang A, & Jin H. (2018). Protective Effect of Colla corii asini against Lung Injuries Induced by Intratracheal Instillation of Artificial Fine Particles in Rats. International Journal of Molecular Sciences, 20(1), 55.
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5

Measuring Mean Arterial Pressure

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Mean arterial pressure (MAP) was determined by catheterization of the femoral artery. The catheter was connected to a pressure transducer (−50, +300 mmHg) and amplifier (Emka Technologies, Paris, France). MAP was calculated using the values of systolic blood pressure (SBP) and diastolic blood pressure (DBP) by the Iox 2.9.5.73 software (Emka Technologies, Paris, France). To calculate a mean arterial pressure, double the diastolic blood pressure and add the sum to the systolic blood pressure. Then divide by 3. MAP = (SBP + 2 (DBP))/3.
Melo B.F., Sacramento J.F., Ribeiro M.J., Prego C.S., Correia M.C., Coelho J.C., Cunha-Guimaraes J.P., Rodrigues T., Martins I.B., Guarino M.P., Seiça R.M., Matafome P, & Conde S.V. (2019). Evaluating the Impact of Different Hypercaloric Diets on Weight Gain, Insulin Resistance, Glucose Intolerance, and its Comorbidities in Rats. Nutrients, 11(6), 1197.
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6

Autonomic Nervous System Balance Assessment

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The balance between the sympathetic and parasympathetic components of the autonomic nervous system was made by calculating the sympathetic nervous system (SNS) and parasympathetic nervous system (PNS) indexes computed in Kubios HRV software (www.kubios.com). The SNS index in Kubios is based on Mean heart rate, Baevsky’s stress index, and low frequency power expressed in normalized units and the PNS index which is based on the mean intervals between successive heartbeats (RR intervals), the root mean square of successive RR interval differences (RMSSD) and high frequency power expressed in normalized units. Heart rate and RR intervals were obtained using Iox 2.9.5.73 software (Emka Technologies, Paris, France), with an acquisition frequency of 500 Hz.
Melo B.F., Sacramento J.F., Ribeiro M.J., Prego C.S., Correia M.C., Coelho J.C., Cunha-Guimaraes J.P., Rodrigues T., Martins I.B., Guarino M.P., Seiça R.M., Matafome P, & Conde S.V. (2019). Evaluating the Impact of Different Hypercaloric Diets on Weight Gain, Insulin Resistance, Glucose Intolerance, and its Comorbidities in Rats. Nutrients, 11(6), 1197.
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