γ ggt activity colorimetric assay kit

Manufactured by Merck Group

The γ-GGT activity colorimetric assay kit is a laboratory tool used to measure the activity of the enzyme gamma-glutamyl transferase (γ-GGT) in biological samples. The kit utilizes a colorimetric method to determine the enzymatic activity, providing a quantitative assessment of γ-GGT levels.

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Lab products found in correlation

Cc 2553, by Lonza (1 mentions) Rptec, by Lonza (1 mentions)

2 protocols using γ ggt activity colorimetric assay kit

Renal Proximal Tubule γ-GGT Assay

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iNephLOs, primary non-neoplastic human renal proximal tubular epithelial cells (RPTEC; Lonza, #CC-2553), hESCs (Day0), and corresponding control cell organoids (shown as Control) were homogenized in 200 μL of ice-cold GGT Assay Buffer. γ-GGT activity was determined using γ-GGT activity colorimetric assay kit (Sigma, MAK089) according to the manufacturer’s instructions.

Hiratsuka K., Monkawa T., Akiyama T., Nakatake Y., Oda M., Goparaju S.K., Kimura H., Chikazawa-Nohtomi N., Sato S., Ishiguro K., Yamaguchi S., Suzuki S., Morizane R., Ko S.B., Itoh H, & Ko M.S. (2019). Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors. Scientific Reports, 9, 913.

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Colorimetric Assay for Renal γ-GGT

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KSP-positive/TRA1-60 negative cells derived from KhES-1 and primary non-neoplastic human renal proximal tubular epithelial cells (RPTEC; Lonza, Cologne, Germany) (1 × 10⁶) were homogenized in 200 μL ice-cold GGT Assay Buffer. γ-GGT activity colorimetric assay kit (MAK089; Sigma-Aldrich) was used according to the manufacturer’s protocol.

Yamaguchi S., Morizane R., Homma K., Monkawa T., Suzuki S., Fujii S., Koda M., Hiratsuka K., Yamashita M., Yoshida T., Wakino S., Hayashi K., Sasaki J., Hori S, & Itoh H. (2016). Generation of kidney tubular organoids from human pluripotent stem cells. Scientific Reports, 6, 38353.

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