1260 infinity lc ms instrument

Chemicals were purchased from the following suppliers: Sinopharm, Adamas and Sigma Aldrich. Solvents were dried before use, if required. Air- and moisture-sensitive reactions were carried out under nitrogen atmosphere. Room temperature (rt) refers to 20–25 °C. The progress of a reaction was monitored by thin layer chromatography (TLC) using pre-coated TLC sheets purchased from Sinopharm. Detected spots were observed under UV light at λ 254 nm and 365 nm. Melting points were measured on a SGW X-4 microscopy melting point apparatus without correction. ¹H spectral data were recorded with a Varian Mercury Plus 400 MHz spectrometer and ¹³C-NMR spectral data were recorded with a Bruker DRX 600MHz spectrometer, both at 303 K using TMS as an internal standard. All chemical shifts were reported in ppm (δ) and coupling constants (J) were in hertz (Hz). Mass spectra were recorded on Agilent Technologies 1260 infinity LC/MS instrument. The chromatograms were conducted on silica gel (100−200 mesh) and visualized under UV light at λ 254 and 365 nm (Supplementary Materials).

Chemicals were purchased from the following suppliers: Sinopharm, Adamas, Merck, and Sigma Aldrich. Solvents were dried before use, if required. Air- and moisture-sensitive reactions were carried out under nitrogen atmosphere. Room temperature (r.t.) refers to 20–25 °C. The progress of a reaction was monitored by thin layer chromatography (TLC) using precoated TLC sheets purchased from Sinopharm. Detected spots were observed under UV light at λ 254 and 365 nm. Melting points were measured on a SGW X-4 microscopy melting point apparatus without correction. ¹H-NMR and ¹³C-NMR spectral data (Supplementary Materials) were recorded with a Bruker DRX 600MHz spectrometer, both at 303 K using TMS as an internal standard. All chemical shifts are reported in ppm (δ) and coupling constants (J) are in hertz (Hz). Mass spectra were recorded on Agilent Technologies 1260 infinity LC/MS instrument. The chromatograms were conducted on silica gel (100–200 and 300–400 mesh) and visualized under UV light at λ 254 and 365 nm.