Indole 3 acetic 2 2 d2 acid

Acetylcholine, acetyl-d3-L-carnitine hydrochloride, acetyl-Lcarnitine, adrenic acid, L-carnitine, Lcitrulline, dodecanoic acid, (-)-epicatechin, gallic acid, glycochenodeoxycholic acid, glycocholic acid, glycocholic acid-(glycyl-1-13C) monohydrate, α-hydroxyisobutyric acid, indole-3-acetic-2,2-d2 acid, Lisoleucine, 7-ketocholesterol, L-leucine, leukotriene B4, margaric acid, palmitic acid, Lphenylalanine, stearic acid, syringic acid, L-tryptophan, uric acid, and L-valine were purchased from Sigma-Aldrich (St. Louis, MO). 4-hydroxyhippuric acid was purchased from PhytoLab GmbH and Co KG (Vestenbergsgreuth, Germany), naringenin from Extrasynthèse (Genay, France), and arachidonic acid from Cymit Quimica (Barcelona, Spain). UHPLC-MS-grade methanol, acetone, formic acid, and HPLC-grade acetonitrile were purchased from Scharlau Chemie S.A. (Barcelona, Spain). Ultrapure water (Milli-Q) was obtained from a Milli-Q Gradient A10 system (Millipore, Bedford, MA).

Internal standard mix of glycocholic acid-(glycine 1-13 C) and 1-O-stearoyl-sn-glycero-3phosphocholine (negative control); external standards mix of acetyl-d3-L-carnitine, indole-3acetic-2,2-d2 acid (Sigma-Aldrich, St Louis, MO) and QC metabolite standard mix were prepared as previously described 20 .
2-hydroxy-hexanoic acid, 3-(4-hydroxyphenyl)-2hydroxypropanoic acid, 3-(4-hydroxyphenyl)-propanoic acid, 3-hydroxy-dodecanoic acid, 3hydroxy-octanoic acid, 3-hydroxy-tetradecanoic acid, 4-hydroxy-butyric acid, acetylcarnitine, caffeine, choline, citric acid, creatine, docosapentaenoic acid (osbond acid), docosatetraenoic acid (adrenic acid), dodecanoic acid (lauric acid), gluconic acid, hexoses, indole-3carboxaldehyde, indolelactic acid, isocitric acid, L-arginine, L-citrulline, L-glutamate, Lhistidine, L-leucine, L-ornithine, L-tryptophan, L-tyrosine, octadecatrienoic acid (linolenic acid), palmitoylmonoglyceride, phe-phe, retinol, tetradecanoic acid (miristic acid), theobromine and uric acid (Sigma, St. Louis, MO) and octadecenoylcarnitine and octadecadienylcarnitine (Larodan, Solna, Sweden) were used to confirm the identity of metabolites.