Anti syntaxin 1a

Mouse antibody anti-syntaxin 1A (1:5000; S0664; HPC-1) and rabbit antibody anti-APP (1:3000; A8717) were obtained from MilliporeSigma (Burlington, MA); rabbit antibody anti-SNAP25 (1:2000; 14903-1-AP), anti-NPTX2 (10889-1-AP), mouse antibodies anti-synaptophysin (1:5000; 67864-1-Ig), anti-β-actin (1:10,000; 60008-1-Ig) were from Proteintech (Rosemont, IL). Mouse antibody anti-synaptobrevin 2 (1:5000; 104 211), rabbit anti-neurofilament L (171 002), and anti-syntaxin 1B (110 402) were purchased Synaptic Systems (Goettingen, Germany). Mouse antibody anti-synaptotagmin (1:2000; 610434) was from BD Biosciences (Franklin Lakes, NJ). Rabbit antibodies anti-synapsin 1 (1:5000; 5297) and anti-synaptojanin 1 (1:1000; 80377) were got from Cell Signaling Technology (Danvers, MA). Mouse antibody anti-SV2A (1:5000; sc-376234) was obtained from Santa Cruz Biotechnology (Dallas, TX). Mouse antibody anti-PSD95 (1:2000; 7E3-1B8) was from ThermoFisher Scientific (Waltham, MA). In all cases, a single band or in some cases doublet, at the expected molecular weight was quantitated. Bands at other molecular weights, of uncertain identify, were detected with some antibodies; they were usually of lesser intensity and were not included in our analysis.