Hs819 t

Manufactured by American Type Culture Collection

Sourced in United States

The Hs819.T is a cell line derived from human lung carcinoma. It is a standardized laboratory tool used for research and development purposes.

Automatically generated - may contain errors

Lab products found in correlation

Sw1353, by American Type Culture Collection (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Atdc5, by RIKEN Cell Bank (1 mentions) 293t cell line, by National Collection of Authenticated Cell Cultures (1 mentions) Nutridoma sp, by Roche (1 mentions) Jj012, by American Type Culture Collection (1 mentions)

3 protocols using hs819 t

Chondrosarcoma and Chondrocyte Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human chondrosarcoma cell lines SW1353 and Hs819.T were purchased from American Type Culture Collection (Manassas, VA, USA) and the mouse chondrogenic cell line ATDC5 was purchased from RIKEN Cell Bank (Tsukuba, Japan). The 293T cell line was purchased from the China Center for Type Culture Collection (Wuhan, China). SW1353 and ATDC5 were cultured in Dulbecco’s modified Eagle medium (DMEM)/F-12 medium (HyClone, Logan City, UT, USA) supplemented with 10% fetal bovine serum (FBS) (HyClone). Hs819.T and 293 T cells were cultured in DMEM medium (HyClone) supplemented with 10% FBS.
Mouse primary chondrocytes were isolated from the articular cartilage of 12-week-old C57BL/6 mice. Human ACs were obtained from ScienCell (Carlsbad, CA, USA). Both mouse and human chondrocytes were cultured in DMEM/F-12 supplemented with 10% FBS.

Lian C., Wang X., Qiu X., Wu Z., Gao B., Liu L., Liang G., Zhou H., Yang X., Peng Y., Liang A., Xu C., Huang D, & Su P. (2019). Collagen type II suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1−SMAD1 interaction. Bone Research, 7, 8.

+ Open protocol

+ Expand

Shear Stress Effects on Chondrosarcoma Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human cell lines derived from chondrosarcoma grade II (SW1353 and HS 819.T obtained from American Type Culture Collection, Manassas, VA, USA) and chondrosarcoma grade III (CH2879 kindly provided by Dr Judith Bovée) were grown (37°C in 5% CO₂) on glass slides in the appropriate medium supplemented with 10% fetal bovine serum. Before exposure to fluid shear stress, cells were incubated for 18 h in serum-free medium supplemented with 1% Nutridoma-SP (Roche Applied Science; Indianapolis, IN, USA).^{18 (link),43 (link),44 (link)} Cells were subjected to shear stress for up to 48 h in medium containing 1% Nutridoma-SP using a streamer gold flow device.^{19 (link),20 (link)} In select experiments, pharmacological agents or blocking antibodies were added to the medium at the indicated concentrations just before the onset of shear or static exposure.
Transient transfection, quantitative real-time reverse transcription PCR, western blot and casein zymography assays were performed as previously described by Zhu et al.^{19 (link)} and Wang et al.^{20 (link)} IGF-2, VEGF-B and VEGF-D levels in static and sheared media were measured using enzyme immunoassay kits.

Wang P., Chen S.H., Hung W.C., Paul C., Zhu F., Guan P.P., Huso D., Kontrogianni-Konstantopoulos A, & Konstantopoulos K. (2014). Fluid shear promotes chondrosarcoma cell invasion by activating matrix metalloproteinase 12 via IGF-2 and VEGF signaling pathways. Oncogene, 34(35), 4558-4569.

+ Open protocol

+ Expand

Culturing Chondrosarcoma and Chondrocyte Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human chondrosarcoma JJ012, Hs 819.T and SW1353 cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). In addition, the normal chondrocyte CHON-002 cell line, also derived from human bone tissue, was purchased from Shanghai Yu Bo Biological Technology Co., Ltd (http://www.sh-ybio.com/). These cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) in a humidified atmosphere of 5% CO₂ at 37°C.

Liu B., Song X., Yan Z., Yang H., Shi Y, & Wu J. (2018). MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1. Oncology Letters, 17(1), 781-788.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!