The MCF7 breast cancer cell line, HCT 116 colorectal carcinoma cell line, A-704 kidney adenocarcinoma cell line, and WI-26 VA4 lung epithelial-like cells were purchased from the ATCC. The MCF7 cells were maintained in MEM (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum (FBS, Gibco, UK), human recombinant insulin (0.01 mg/mL), penicillin (100 UI mL−1), streptomycin (100 µg mL−1), and GlutaMax (2 mM, Gibco, UK). The HCT 116 cells were maintained in McCoy’s 5A (Gibco, UK) supplemented with 10% fetal bovine serum (FBS, Gibco, UK), penicillin (100 UI mL−1), streptomycin (100 µg mL−1), and GlutaMax (1.5 mM, Gibco, UK). A-704 and WI-26 VA4 cells were maintained in MEM (Gibco, UK) supplemented with 10% fetal bovine serum (FBS, Gibco, UK), penicillin (100 UI mL−1), streptomycin (100 µg mL−1), and GlutaMax (1.87 mM, Gibco, UK). All cell lines’ cultivation was performed under a humidified atmosphere of 95% air/5% CO2 at 37 °C. Subconfluent monolayers, in the log growth phase, were harvested by a brief treatment with TrypLE Express solution (Gibco, UK) in phosphate-buffered saline (PBS, Capricorn Scientific, Ebsdorfergrund, Germany) and washed three times in serum-free PBS. The number of viable cells was determined by trypan blue exclusion.
Wi 26 va4
Manufactured by American Type Culture Collection
Sourced in United States
The WI-26 VA4 is a cell line derived from the lung tissue of a human fetal specimen. It is a valuable tool for research in various fields, including virology, cell biology, and pharmaceutical development. The cell line exhibits stable growth characteristics and can be used for various in vitro experiments and assays.
Automatically generated - may contain errors
Lab products found in correlation
Mrc 5, by American Type Culture Collection (3 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Tryple express solution, by Thermo Fisher Scientific (2 mentions)
Gammacell 40 exactor, by Nordion (2 mentions)
Nci h292, by American Type Culture Collection (2 mentions)
Mcf 7 breast cancer cell line, by American Type Culture Collection (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (1 mentions)
Advanced mem, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hcc44, by American Type Culture Collection (1 mentions)
Nci h838, by American Type Culture Collection (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
Tov 21g, by American Type Culture Collection (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Bt 549, by Merck Group (1 mentions)
7 protocols using wi 26 va4
1
Cell Culture Maintenance Protocol
2
Cell Culture Protocols for Lung Cancer and Control Cells
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A549, NCI-H460, NCI-H1975 lung cancer cells and WI-26 VA4 lung epithelial-like cells were purchased from the ATCC. A549 cells were maintained in F12-K (Corning, NY, USA) supplemented with 10% fetal bovine serum (Fetal Bovine Serum, qualified, Australia; Gibco, Loughborough, UK), penicillin (100 UI mL−1), streptomycin (100 µg mL−1), and GlutaMax (1.9 mM, Gibco, UK). NCI-H460 and NCI-H1975 cells were maintained in RPMI-1640 (ATCC modification) media (Gibco, Loughborough, UK) supplemented with 10% fetal bovine serum (Fetal Bovine Serum, qualified, Australia; Gibco, Loughborough, UK), penicillin (100 UI mL−1), streptomycin (100 µg mL−1), and GlutaMax (2 mM, Gibco, UK). WI-26 VA4 cells were maintained in Advanced MEM (Gibco, Loughborough, UK) supplemented with 5% fetal bovine serum (Fetal Bovine Serum, qualified, Australia, Gibco, UK), penicillin (100 UI mL−1), streptomycin (100 µg mL−1), and GlutaMax (1.87 mM, Gibco, Loughborough, UK). All cells line cultivation under a humidified atmosphere of 95% air/5% CO2 at 37 °C. Subconfluent monolayers, in the log growth phase, were harvested by a brief treatment with TrypLE Express solution (Gibco, Loughborough, UK) in phosphate buffered saline (PBS, Capricorn Scientific, Germany) and washed three times in serum-free PBS. The number of viable cells was determined by trypan blue exclusion.
3
Culturing Lung Cancer Cell Lines
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Lung cancer cell lines A427, A549, HCC44, and normal lung fibroblast cell lines WI-26 VA4 and MRC-5, were obtained from the American Type Culture Collection (ATCC). All cells were cultured in DMEM (Gibco, USA), 10% (v/v) fetal bovine serum and with 100 Upenicillin and streptomycin, and were maintained in a humidified atmosphere, 95% air, 5% CO2 at 37°C.
4
Radiation Exposure in NSCLC Cell Lines
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The human NSCLC cell lines, NCI-H292 and NCI-H838, and the human normal lung cell lines, MRC5 and WI-26 VA4, were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA), authenticated and maintained in early passages, no more than 6 months after receipt from ATCC. NCI-H292, NCI-H838, MRC5 or WI-26 VA4 cells were grown in RPMI-1640 or Dulbecco's modified Eagle's medium supplemented with 10% FBS, 100 U ml−1 penicillin and 100 mg ml−1 streptomycin at 37 °C in a 5% CO2/95% air atmosphere. Cells were exposed to a single dose of γ-rays using a Gamma Cell 40 Exactor (Nordion International, Kanata, ON, Canada) at a dose of 0.81 Gy min−1. Flasks containing control cells were placed in the irradiation chamber, but not exposed to radiation.
5
Cell Culture Conditions for Cancer Lines
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Cell lines BT-549 (breast ductal carcinoma; HTB-122™), TOV-21G (ovarian adenocarcinoma; CRL-11730™), RKO-AS45-1 (colorectal carcinoma; CRL-2579™) and WI-26 VA4 (lung fibroblast used as a control; CCL-95.1™) were purchased from the American Type Culture Collection. The BT-549 cell line was cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) inactivated at 56°C for 30 min, 1% 0.2 M L-glutamine and 10 mg/ml bovine insulin (Sigma-Aldrich; Merck KGaA). The TOV-21G cell line was grown in high-glucose Dulbecco's modified Eagle's medium (Sigma-Aldrich; Merck KGaA) supplemented with 15% FBS and 1% 0.2 M L-glutamine, and the RKO-AS45-1 and WI-26 VA4 cell lines were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% 0.2 M L-glutamine. Cells were incubated at 37°C in a humidified atmosphere enriched with 5% CO2. The experiments were carried out obeying a certain passage number (between passages 4 and 6).
6
Cell Transfection and Irradiation Protocol
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MRC5 and WI-26 VA4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), authenticated, and maintained in early passages, no more than 6 months after receipt from ATCC. The cells were cultured in DMEM medium containing 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 95% air/5% CO2. For transient transfection, cells were plated at a density of 6 × 105 cells in 60 mm dishes and incubated for 24 h. The cells were transiently transfected with siRNAs adequate for the experiments. The media was changed with fresh media after 6 h transfection and subsequent treatment or harvested is performed. For treatment, transfected or non-treated cells with 80% confluence were administrated with MG132 (10 μM) dissolved in dimethyl sulfoxide (DMSO) or Chloroquine (100 μM) dissolved in distilled water. After 30 min of incubation, the cells were irradiated with 2 Gy of γ-ray by using a Gamma cell 40 Exactor (Nordion International, Inc. Kanata, Ontario, Canada). The cells were utilized in subsequent experiments after 4 h after irradiation.
7
Cell Line Maintenance and Authentication
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The human normal lung cell line, WI-26 VA4, and four human NSCLC cell lines, A549, NCI-H292, NCI-H358, and NCI-H460, were acquired from the American Type Culture Collection (ATCC, Manassas, VA), authenticated, and then maintained in early passages for no more than 6 months after receipt from ATCC. The WI-26 VA4, A549, NCI-H460, NCI-H292, and NCI-H358 cells were grown in DMEM or RPMI-1640 supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a 5% CO2/95% air atmosphere.
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