Araldite cy212

Commercial formulation of the glyphosate herbicide (Excel Mera 71, Excel Crop Care Limited) was used in both the experiments. Excel Mera 71 is the trade name of glyphosate herbicide in the Indian Market. Delafield's hematoxylin stain, eosin yellow, xylene, Distyrene Plasticizer xylene, amyl acetate, acetone, glutaraldehyde solution, sodium hydroxide, tricaine methonesulfonate, uranyl acetate (EM grade), ethanol, disodium hydrogen phosphate, dihydrogen sodium phosphate, lead citrate (EM grade), epoxy resin (EM grade), paraformaldehyde (EM grade), and araldite CY212 (EM grade) of analytical grade were purchased from Merck Specialities Private Limited (Mumbai, India). Osmium tetroxide was purchased from Spectrochem (Mumbai, India).

Specimens of trachea were fixed by immersion in 4% glutaraldehyde (Merck) in 0.1 M Sorensen phosphate buffer solution (SPBS; pH 7.2) for 4 hours and post-fixed in 1% SPBS buffered osmium tetroxide (OsO4) for 2 hours. Samples were dehydrated in an ascending graded ethanol series and were embedded in Araldite CY212 (Merck). Semithin sections were prepared using an ultramicrotome (Ultratome NOVA-LKB), counterstained with 1% toluidine blue, and examined with a Zeiss Axioplan light microscope (Jena, Germany). To qualitatively evaluate decellularized matrix structure, matrices were fixed with 4% (v/v) glutaraldehyde in a buffered solution of 0.1 M SPBS (pH 7.2). After rinsing in Sorensen phosphate, specimens were dehydrated thr-ough an ethanol gradient, critical point dried (Polaron, E3000; Quorum Technologies, West Sussex, UK), sputter coated (Polaron, SC7620) with gold and analyzed using Zeiss LEO 1430 sca-nning electron microscope (SEM; Oberkochen, Germany).

Commercial formulation of the Almix herbicide (Almix^® 20 WP, DuPont India Pvt. Ltd., Gurgaon, Haryana, India) was used in both the experiments. Delafield’s hematoxylin stain, eosin yellow, xylene, DPX, amyl acetate, acetone, glutaraldehyde solution, sodium hydroxide, tricaine methanesulphonate, uranyl acetate (EM grade), ethanol, disodium hydrogen phosphate, dihydrogen sodium phosphate, lead citrate (EM grade), epoxy resin (EM grade), paraformaldehyde (EM grade) and araldite CY212 (EM grade) of analytical grade were purchased from Merck Specialities Private Limited. Osmium tetraoxide was purchased from Spectrochem Pvt. Ltd., Mumbai, India.