Rabbit anti paxillin

Manufactured by GeneTex

Rabbit anti-paxillin is a primary antibody that specifically binds to the paxillin protein. Paxillin is a focal adhesion adapter protein involved in the regulation of cell adhesion and cytoskeleton organization. This antibody can be used for the detection and analysis of paxillin in various applications, such as Western blotting, immunohistochemistry, and immunoprecipitation.

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4 protocols using rabbit anti paxillin

Immunofluorescence and Western Blot Analysis

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Mouse anti-SAS6, rabbit anti-cortactin, and mouse anti-γ-tubulin: Santa Cruz; rabbit anti-STIL and mouse anti-TRIO: Abcam; rabbit anti-GEF-H1 and rabbit anti-integrin β1 (total form), mouse anti-GAPDH, rabbit anti-GOLPH2, rabbit anti-β-actin, rabbit anti-paxillin, mouse anti-γ-tubulin, and rabbit anti-NMIIA: GeneTex; mouse anti-α-tubulin and mouse anti-acetylated tubulin: Sigma-Aldrich; rat anti-α-tubulin: ABD; mouse anti-paxillin: BD; mouse anti-Rac1 and mouse anti-integrin β1 (active form): Millipore; rabbit anti-TRIO, Alexa Fluor 488 phalloidin, Alexa Fluor 488-anti-rat IgG, Alexa Fluor 488-anti-rabbit IgG, Alexa Fluor 488-anti-mouse IgG, Alexa Fluor 568-anti-mouse IgG, Alexa Fluor 568-anti-rabbit IgG, Alexa Fluor 680-anti-rabbit IgG, and DAPI: Thermo Fisher; and HRP-AffiniPure mouse anti-rabbit IgG and HRP-AffiniPure goat anti-mouse IgG: Jackson ImmunoResearch.

Cheng H.W., Hsiao C.T., Chen Y.Q., Huang C.M., Chan S.I., Chiou A, & Kuo J.C. (2019). Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration. Life Science Alliance, 2(1), e201800135.

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Integrin-Mediated Cellular Adhesion Analysis

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Mouse anti-paxillin (BD 610052); rat anti-active integrin β1 (9EG7; BD 553715); rabbit-anti-Tom20 (Santa Cruz sc-11415); mouse-anti-MYH1/2 (Santa Cruz sc-53088); rabbit anti-fibronectin (Santa Cruz sc-9068); rabbit anti-GAPDH (GeneTex GTX100118); rabbit anti-β-actin (GeneTex GTX100313); rabbit anti-integrin β1 (GeneTex GTX128839); rabbit anti-paxillin (GeneTex GTX125891); rabbit anti-pY397-FAK (GeneTex GTX129840); rabbit anti-kinesin-1 (Abcam AB167429); rabbit anti-GFP (Abcam AB290); mouse anti-α-tubulin (Sigma T5168); mouse anti-MYH2 (Thermo Fisher 14-6503-80); rabbit anti-FAK (Thermo Fisher AHO0502); Alexa Fluor 488 phalloidin (Thermo Fisher A12379); Alexa Fluor 568 phalloidin (Thermo Fisher A12380); Alexa Fluor 488-anti-rabbit IgG (Thermo Fisher A11034); Alexa Fluor 488-anti-mouse IgG (Thermo Fisher A11029); Alexa Fluor 488-anti-rat IgG (Thermo Fisher A11006); Alexa Fluor 568-anti-rabbit IgG (Thermo Fisher A11036); Alexa Fluor 568-anti-mouse IgG (Thermo Fisher A11031); DAPI (Thermo Fisher D1306); Mitotracker Red (Thermo Fisher M7512); HRP-AffiniPure mouse anti-rabbit IgG (Jackson ImmunoResearch 211-032-171); and HRP-AffiniPure goat anti-mouse IgG (Jackson ImmunoResearch 115-035-174).

Lin J.W., Huang Y.M., Chen Y.Q., Chuang T.Y., Lan T.Y., Liu Y.W., Pan H.W., You L.R., Wang Y.K., Lin K.H., Chiou A, & Kuo J.C. (2021). Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals. Cell Death Discovery, 7, 35.

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Western Blot Analysis of Bladder Lysates

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Whole cell lysates from 5637 were prepared for western blot as previously described [39 (link)]. The following antibodies were used: rabbit anti-HIF-1α antibody, rabbit anti-HIF-2α (NB100-122) (Novus Biological), mouse anti-P84 antibody (Catalog No. GTX70220) (GeneTex), rabbit anti-paxillin, rabbit anti-phospho-p65 (Ser536) monoclonal antibody (Catalogue No. 3033) and rabbit anti-phospho-p38 (Thr180/Tyr182) polyclonal antibody (Catalogue No. 92122) (Cell Signaling Technologies). Bladder homogenates were prepared by lysing a bladder in 500 μL RIPA lysis buffer with beads using the MegaLyser (Roche). Cathelicidin detection was made using the same anti-cathelicidin antibody as immunolocalization. Anti-mouse β-actin monoclonal antibody clone AC-74 (Catalogue No. A5316, Sigma-Aldrich) was used as a loading control. Band intensity was measured using Image J software.

Lin A.E., Beasley F.C., Olson J., Keller N., Shalwitz R.A., Hannan T.J., Hultgren S.J, & Nizet V. (2015). Role of Hypoxia Inducible Factor-1α (HIF-1α) in Innate Defense against Uropathogenic Escherichia coli Infection. PLoS Pathogens, 11(4), e1004818.

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Immunofluorescence Microscopy of Focal Adhesions

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All reagents were acquired from Sigma-Aldrich (St. Louis, MO) unless otherwise specified. Primary antibodies used for immunofluorescence microscopy were mouse anti-vinculin (hVin-1, Sigma; 1:200), rabbit anti-paxillin (GeneTex, Irvine, CA; 1:200), mouse anti-c-myc (9B11, Cell Signalling Technologies, Danvers, MA; 1:200). Alexa Flour 680-conjugated streptavidin was from Life Technologies, and secondary antibodies (anti-mouse IgG Alexa Flour 488 and anti-rabbit IgG Alexa Flour 488) were from Invitrogen (Carlsbad, CA).

Chastney M.R., Lawless C., Humphries J.D., Warwood S., Jones M.C., Knight D., Jørgensen C., & Humphries M.J. (2020). Definition of the fibroblast adhesome using multiplexed proximity biotinylation.

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