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Free glycerol detection reagent

Manufactured by Merck Group
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Free glycerol detection reagents are laboratory products designed to quantify the concentration of free glycerol in a sample. These reagents provide a colorimetric or fluorometric method to measure the amount of free glycerol present, which can be useful in various biochemical and analytical applications.

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Lab products found in correlation

Phosphatidylinositol, by Merck Group (2 mentions) Phosphatidylcholine, by Merck Group (2 mentions) Triolein, by Merck Group (2 mentions) 1 rac oleoylglycerol, by Merck Group (2 mentions) Oleoyl coa, by Merck Group (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Nefa kit, by Fujifilm (1 mentions) Hi 76 0079, by Novo Nordisk (1 mentions) 1 oleoyl 2 hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Protein assay kit, by Bio-Rad (1 mentions) Columns for protein purification, by GE Healthcare (1 mentions)

4 protocols using free glycerol detection reagent

1

Triolein Metabolism Assay Protocol

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[9,10-3H]Triolein was obtained from PerkinElmer Life Sciences. Triolein, phosphatidylcholine, phosphatidylinositol, 1(rac)-oleoylglycerol, oleoyl-CoA, and free glycerol detection reagents were purchased from Sigma. 1-Oleoyl-2-hydroxy-sn-glycero-3-phosphocholine was purchased from Avanti Polar Lipids Inc., Alabaster, AL, and the NEFA kit was from WAKO Diagnostics, Neuss, Germany. Hi76-0079 obtained from Novo Nordisk, Denmark, Atglistatin was a generous gift from R. Breinbauer (Graz University of Technology, Austria). The protein assay kit was obtained from Bio-Rad; Thermo Scientific, Rockford, IL was the source for the Pierce® BCA protein assay kit. The synthetic peptides were synthesized by Peptide Specialty Laboratories GmbH, Heidelberg, Germany.
Cerk I.K., Salzburger B., Boeszoermenyi A., Heier C., Pillip C., Romauch M., Schweiger M., Cornaciu I., Lass A., Zimmermann R., Zechner R, & Oberer M. (2014). A Peptide Derived from G0/G1 Switch Gene 2 Acts as Noncompetitive Inhibitor of Adipose Triglyceride Lipase. The Journal of Biological Chemistry, 289(47), 32559-32570.
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2

Triolein Metabolism Analysis Protocol

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If not stated otherwise, chemicals, antibiotics, and buffers were obtained from Sigma-Aldrich (St. Louis, MO) or Carl Roth GmbH (Karlsruhe, Germany); columns for protein purification were from GE Healthcare Life Sciences (Chicago, IL). The [9,10-3H] Triolein was obtained from PerkinElmer Life Sciences (Waltham, MA). Triolein, phosphatidylcholine, phosphatidylinositol, 1(rac)-oleoylglycerol, oleoyl-CoA, and free glycerol detection reagents were purchased from Sigma-Aldrich.
Padmanabha Das K.M., Wechselberger L., Liziczai M., De la Rosa Rodriguez M., Grabner G.F., Heier C., Viertlmayr R., Radler C., Lichtenegger J., Zimmermann R., Borst J.W., Zechner R., Kersten S, & Oberer M. (2018). Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase. Journal of Lipid Research, 59(3), 531-541.
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3

Glycerol Detection in Adipocytes

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Glycerol assays were performed using free glycerol detection reagent (Sigma, F6428) as per manufacturer’s instructions. For serum glycerol, terminal blood collections were performed using cardiac puncture method and sera were frozen immediately at −80C until use. For glycerol detection, 1:20 ratio of sera to free glycerol reagent was used to perform the assay.
For in vitro differentiated adipocytes, wild-type and Opn3 null cells were dark adapted overnight on Day13 or Day14 of differentiation and serum starved for at least 3 hours on the day of the experiment. All cells were given fresh serum-free media before stimulating one set of wild-type and Opn3 null cells with blue light, while another set of cells were left in the dark incubator. Culture media was collected at the end of 2 hours of incubation in blue light or darkness and frozen on dry ice immediately for storage at −80C until use. For the glycerol assay, a 1:10 ratio of culture media to free glycerol reagent was used to determine the amount of glycerol released during the 2 hours of darkness or blue light stimulation.
Nayak G., Zhang K.X., Vemaraju S., Odaka Y., Buhr E.D., Holt-Jones A., Kernodle S., Smith A.N., Upton B.A., D’Souza S., Zhan J.J., Diaz N., Nguyen M.T., Mukherjee R., Gordon S.A., Wu G., Schmidt R., Mei X., Petts N.T., Batie M., Rao S., Hogenesch J.B., Nakamura T., Sweeney A., Seeley R.J., Van Gelder R.N., Sanchez-Gurmaches J, & Lang R.A. (2020). Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light Sensing. Cell reports, 30(3), 672-686.e8.
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4

Glycerol Detection in Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glycerol assays were performed using free glycerol detection reagent (Sigma, F6428) as per manufacturer’s instructions. For serum glycerol, terminal blood collections were performed using cardiac puncture method and sera were frozen immediately at −80C until use. For glycerol detection, 1:20 ratio of sera to free glycerol reagent was used to perform the assay.
For in vitro differentiated adipocytes, wild-type and Opn3 null cells were dark adapted overnight on Day13 or Day14 of differentiation and serum starved for at least 3 hours on the day of the experiment. All cells were given fresh serum-free media before stimulating one set of wild-type and Opn3 null cells with blue light, while another set of cells were left in the dark incubator. Culture media was collected at the end of 2 hours of incubation in blue light or darkness and frozen on dry ice immediately for storage at −80C until use. For the glycerol assay, a 1:10 ratio of culture media to free glycerol reagent was used to determine the amount of glycerol released during the 2 hours of darkness or blue light stimulation.
Nayak G., Zhang K.X., Vemaraju S., Odaka Y., Buhr E.D., Holt-Jones A., Kernodle S., Smith A.N., Upton B.A., D’Souza S., Zhan J.J., Diaz N., Nguyen M.T., Mukherjee R., Gordon S.A., Wu G., Schmidt R., Mei X., Petts N.T., Batie M., Rao S., Hogenesch J.B., Nakamura T., Sweeney A., Seeley R.J., Van Gelder R.N., Sanchez-Gurmaches J, & Lang R.A. (2020). Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light Sensing. Cell reports, 30(3), 672-686.e8.
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