Western blot was used to detect the expression of different proteins in RAW264.7 and BMSCs. Anti-c-Fos (Beyotime, 1:2000), anti-NFATc1(ABclonal, 1:3000), anti-TRAP(Abcam, 1:5000), anti-cathepsin-K(Abcam, 1:2000), anti-TLR4(Sangon Biotech, 1:2000), anti-p-p65(Beyotime, 1:2000), anti-p65(ABclonal, 1:2000), anti-p-IκBα(ImmunoWay, 1:2000), anti-IκBα(ImmunoWay, 1:2000), anti-p-ERK (ABclonal, 1:2000), anti-ERK(ABclonal, 1:2000), anti-p-p38(ABclonal, 1:2000), anti-p38 (ABclonal, 1:2000), anti-p-JNK(ABclonal, 1:2000), anti-JNK(Abcam, 1:2000), anti-BMP-2(Abcam, 1:2000), anti-RUNX2(Abcam, 1:2000), anti-β-catenin(Abcam, 1:10,000), anti-p53(Abcam, 1:2000), anti-bax (Abcam, 1:10,000), anti-bcl-2(Abcam, 1:2000), anti-PPAR-γ(Solarbio, 1:2000), anti-β-actin(Abcam, 1:2000), goat anti-mouse IgG(Abcam, 1:5000), goat anti-rabbit IgG(Abcam, 1:5000) were used for protein analysis. ECL substrate kit (BL520B, biosharp) was used for visual analysis of proteins. The gray values of the bands were quantified by ImageJ software.
Anti erk
Manufactured by ABclonal
Sourced in China, United States
Anti-ERK is a lab equipment product designed to detect and quantify the levels of Extracellular Signal-Regulated Kinase (ERK) in biological samples. ERK is a key signaling protein involved in cellular processes such as proliferation, differentiation, and survival. This product can be used to measure ERK expression and activation in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti p erk, by ABclonal (3 mentions)
Anti p65, by ABclonal (2 mentions)
Goat anti rabbit igg, by Abcam (1 mentions)
Anti bax, by Abcam (1 mentions)
Anti p p65, by Beyotime (1 mentions)
Anti jnk, by Abcam (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Anti bcl 2, by Abcam (1 mentions)
Anti p53, by Abcam (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti bmp2, by Abcam (1 mentions)
Anti runx2, by Abcam (1 mentions)
Anti cathepsin k, by Abcam (1 mentions)
Anti nfatc1, by ABclonal (1 mentions)
Anti p p38, by ABclonal (1 mentions)
Anti p38, by ABclonal (1 mentions)
Goat anti mouse igg, by Abcam (1 mentions)
Sds page, by Merck Group (1 mentions)
Anti akt1, by Santa Cruz Biotechnology (1 mentions)
Anti fas, by Abcam (1 mentions)
4 protocols using anti erk
1
Protein Expression Analysis in RAW264.7 and BMSCs
2
Western Blot Analysis of MSC Markers
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MSCs were lysed and the protein concentration was determined using the Pierce BCA Protein Assay Kit. Aliquots (40 μg) of protein solutions were resolved by 10% SDS-PAGE (Millipore, Billerica, MA) and transferred to PVDF membranes. The membranes were incubated with diluted anti-RUNX2, anti-PPARγ, anti-GAPDH, anti-β-catenin (ProteinTech), anti-CD9, anti-CD81, anti-Fas, anti-integrin alpha-5, anti-CD44, anti-p65 (Abcam), anti-calreticulin (Cell Signaling Technology), anti-AKT1 (Santa Cruz), anti-p-AKT1, anti-ERK and anti-p-ERK (Abclonal) primary antibodies, followed by the corresponding secondary antibodies (Cell Signaling Technology). Bands were detected using the ECL Kit (NCM bio, Suzhou, China). Images were analyzed using Image J software (National Institutes of Health, Bethesda, MD).
3
Hericium erinaceus Extract Bioactivity Profiling
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The fruiting body of Hericium erinaceus was purchased from Jilin Jiaohe Songshan Food Co., Ltd. (Jiaohe, China). Dextran standards (670, 410, 270, 80, 25, 12 and 5 kDa) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Monosaccharide standards, including arabinose, mannose, fucose, xylose, rhamnose, galactose and glucose were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Cell Counting Kit-8 was obtained from APExBIO Technology LLC (Houston, TX, USA). Neutral red staining solution, nitric oxide assay kit and BCA kit were obtained from Beyotime Biological Technology Co., Ltd. (Shanghai, China). Western Blot assay kit, SDS-PAGE kit, ELISA kits of TNF-α, IL-6, IL-10 and IFN were obtained from Boster Biological Technology Co., Ltd. (Wuhan, China). Anti-ERK, anti-phospho ERK, anti-JNK, anti-phospho JNK, anti-p38 MAPK, anti-phospho p38 MAPK, anti-Akt, anti-phospho Akt, anti-IkBα, anti-p65, anti-β-actin and anti-Histone H2A were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China). Inhibitors of BAY11-7082, SB203580, SP600125, PD98059 and LY294002 were purchased from Selleck Chemicals (Shanghai, China).
4
Investigating ERK Signaling in Kif22 Knockdown
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SCs transfected with the indicated siRNAs (Kif22-specific siRNAs or negative control siRNA) were lysed with a buffer containing protease and phosphatase inhibitors to extract total protein. The extracted proteins were subjected to electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel and then transferred to a nitrocellulose membrane (Merck Millipore, Billerica, MA, USA). Next, the membranes were incubated with anti-ERK (rabbit, 1:1000, Cat# A4782, Abclonal, Boston, MA, USA), anti-p-ERK (phospho-ERK1-T202/Y204 + ERK2-T185/Y187, rabbit, 1:1000, Cat# AP0974, Abclonal), anti-protein kinase C α (PKCα; rabbit, 1:1000, Cat# A11107, Abclonal), and anti-GAPDH (rabbit, 1:2500, Cat# G9545, Sigma) antibodies at 4°C overnight. Then, the samples were incubated with goat anti-rabbit IgG-horseradish peroxidase (1:1000, Cat# abs20040, Absin Bioscience Inc, Shanghai, China) at room temperature for 2 hours. The blot images were scanned using a Tanon5200multi system (Tanon, Shanghai, China). Finally, quantitative analysis was performed using ImageJ software. Protein levels were normalized to GAPDH.
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